Summary of Study ST000100
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000092. The data can be accessed directly via it's Project DOI: 10.21228/M81S38 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000100 |
| Study Title | Global Metabolomics of C. elegans using an augmented reference IROA design |
| Study Type | heat shock |
| Study Summary | Global Metabolomics of C. elegans using an augmented reference IROA design |
| Institute | University of Florida |
| Department | Biochemistry & Molecular Biology |
| Laboratory | Arthur Edison |
| Last Name | Edison |
| First Name | Arthur |
| Address | R3-226 Academic Research Building, Department of Biochemistry & Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245 |
| aedison@ufl.edu | |
| Submit Date | 2014-09-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 16 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-02-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000165 | AN000166 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units |
MS:
| MS ID: | MS000134 |
| Analysis ID: | AN000165 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Q-Exactive was equipped with a Heated Electrospray Ionization (HESI) source which operated at a spray temperature of 350 ?C, a spray voltage of 3.5 kV, and sheath and auxiliary gas flow rates of 50 and 10 arbitrary units, respectively. The capillary temperature was held at 325 ?C, and the S-lens RF Level was set to 40%. The FR resolution was set to 70,000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm. |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 325 ?C |
| Ion Source Temperature: | 350 ?C |
| Ion Spray Voltage: | 3.5 kV |
| Resolution Setting: | 70000 |
| MS ID: | MS000135 |
| Analysis ID: | AN000166 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The Q-Exactive was equipped with a Heated Electrospray Ionization (HESI) source which operated at a spray temperature of 350 ?C, a spray voltage of 3.5 kV, and sheath and auxiliary gas flow rates of 50 and 10 arbitrary units, respectively. The capillary temperature was held at 325 ?C, and the S-lens RF Level was set to 40%. The FR resolution was set to 70,000 at m/z 200. The accuracy achieved was routinely less than 1.5 ppm. |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 325 ?C |
| Ion Source Temperature: | 350 ?C |
| Ion Spray Voltage: | 3.5 kV |
| Resolution Setting: | 70000 |
