Summary of Study ST000199
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000154. The data can be accessed directly via it's Project DOI: 10.21228/M8688J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000199 |
Study Title | IDH1 and Glioma knockdown idh1 (part II) |
Study Summary | Tumor neurospheres were grown in culture until 90% confluent. To collect cells, cells were filtered through 0.45um nylon filters (Fischer Sci 099029) that were prewashed with methanol and ultrapure water using a microanalytical glass filter holder (Fisher Sci 09753E). The cells were filtered through filtration apparatus, then washed with 1mL of distilled water very quickly. Filter paper is taken immediatedly with tweezers and plunged cell side down into 6-well plate filled with liquid nitrogen placed on dry ice. Plate is wrapped in aluminum foil and stored at -80C before the liquid nitrogen evaporates. Samples are shipped on dry ice |
Institute | University of Michigan |
Department | Biomedical Research Core Facilities |
Laboratory | Metabolomics core |
Last Name | Kachman |
First Name | Maureen |
Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
mkachman@umich.edu | |
Submit Date | 2015-06-10 |
Num Groups | 2 |
Total Subjects | 10 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Uploaded File Size | 16 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-12-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000301 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1200 |
Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | POSITIVE |
Units | pmol/µg protein |
MS:
MS ID: | MS000250 |
Analysis ID: | AN000301 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Acquisition Parameters File: | QM-004-Xbridg2mm_ACar+_MRM-Insert.m.zip |
Processing Parameters File: | EX00311-acyl-carnitines-quant.m.zip |