Summary of Study ST000263

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000263
Study TitleActivity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv1016
Study Typetimecourse
Study SummaryThese experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze.
Institute
Weill Cornell Medicine
DepartmentMicrobiology and Immunology
Last NameRhee
First NameKyu
AddressDepartment of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY
Emailkyr9001@med.cornell.edu
Submit Date2015-11-13
Num Groups3 replicates X 3 timepoints
Study CommentsThe timepoints in the datafiles refer the amount of time (in minutes) that each protein was incubated with lysate.The units for 'mzmed' are mass/charge.
The units for 'rtmed' are seconds (retention time).
The units for all the other data columns in the spreadsheets are arbitrary; they are integrated peak counts.
In this study, Rv1713 was incubated with lysate with and without 1mM GTP added as a co-factor.
Raw Data AvailableNo
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version2
Release CommentsUpdated study design factors
Kyu Rhee Kyu Rhee
https://dx.doi.org/10.21228/M8388M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000419 AN000420
Analysis type MS MS
Chromatography type Normal phase Normal phase
Chromatography system Agilent 1290 Agilent 1290
Column Microsolv Diamond hydride (150mm,4um) Microsolv Diamond hydride (150mm,4um)
MS Type ESI ESI
MS instrument type TOF TOF
MS instrument name Agilent 6220 TOF Agilent 6220 TOF
Ion Mode POSITIVE NEGATIVE
Units integrated peak counts integrated peak counts

MS:

MS ID:MS000360
Analysis ID:AN000419
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:3500
Dry Gas Flow:10 l/min
Dry Gas Temp:250
Fragment Voltage:135V
Mass Accuracy:5 ppm
Nebulizer:35
Octpole Voltage:400
Scan Range Moverz:50-1200
Scanning Cycle:1.4
Skimmer Voltage:65
  
MS ID:MS000361
Analysis ID:AN000420
Instrument Name:Agilent 6220 TOF
Instrument Type:TOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Voltage:3500
Dry Gas Flow:10 l/min
Dry Gas Temp:250
Fragment Voltage:135V
Mass Accuracy:5 ppm
Nebulizer:35
Octpole Voltage:400
Scan Range Moverz:50-1200
Scanning Cycle:1.4
Skimmer Voltage:65
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