Summary of Study ST000372
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000134. The data can be accessed directly via it's Project DOI: 10.21228/M8HP4Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000372 |
| Study Title | Measure change in metabolites based on diet and feeding status (part I) |
| Study Type | Regular |
| Study Summary | Flies were fed a normal diet or high-sugar (30% sucrose) diet for 10 days. Flies of each diet were flash frozen in liquid nitrogen as sated (refed for 3hrs on 400mM D-glucose) or after fasting for 24hrs. Heads and bodies were separated using a sieve-system and placed immediately on dry ice (total time app. 1min). We collected 50 heads per condition (4 conditions total) and 5 biological replicates |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2016-04-18 |
| Num Groups | 4 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000606 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | None |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6410 QQQ |
| Ion Mode | POSITIVE |
| Units | pmol/µg tissue protein |
MS:
| MS ID: | MS000539 |
| Analysis ID: | AN000606 |
| Instrument Name: | Agilent 6410 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | QM-005-Xbrdg2mm-CoA-6490.m.zip |
