Summary of Study ST000401
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000313. The data can be accessed directly via it's Project DOI: 10.21228/M8QG7W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000401 |
Study Title | Inhibition of diamine oxidase promotes uptake of putrescine from rat small intestine |
Study Summary | Metformin, a biguanide molecule, which is used as first line therapy for type 2 diabetes. In this study, we would like to investigate the inhibition of an enzyme called diamine oxidase (DAO) (also known as ABP1), by metformin. Based on our preliminary in vitro study using diamine oxidase enzyme, we saw increased level of putrescine with increasing metformin concentrations (see reference PMID: 26335661). This proposed in vivo study was to determine whether metformin could increase putrescine levels and other metabolites in mice. Aminoguanidine, a known inhibitor of DAO, in this study as positive control, following similar study design described in this paper (PMID: 8912017). |
Institute | University of California, Davis |
Department | Genome and Biomedical Sciences Facility |
Laboratory | WCMC Metabolomics Core |
Last Name | Fiehn |
First Name | Oliver |
Address | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
ofiehn@ucdavis.edu | |
Phone | (530) 754-8258 |
Submit Date | 2016-05-16 |
Num Groups | 5 |
Total Subjects | 40 |
Raw Data Available | Yes |
Raw Data File Type(s) | peg |
Analysis Type Detail | GC-MS |
Release Date | 2016-06-18 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN000640 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Restek Corporation Rtx-5Sil MS |
MS Type | EI |
MS instrument type | GC-TOF |
MS instrument name | Leco Pegasus III GC TOF |
Ion Mode | POSITIVE |
Units | counts |
MS:
MS ID: | MS000572 |
Analysis ID: | AN000640 |
Instrument Name: | Leco Pegasus III GC TOF |
Instrument Type: | GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |
Ion Source Temperature: | 250 C |
Ionization Energy: | 70 eV |
Mass Accuracy: | Nominal |
Source Temperature: | 250 C |
Scan Range Moverz: | 85-500 Da |
Scanning Cycle: | 17 Hz |
Scanning Range: | 85-500 Da |
Skimmer Voltage: | 1850 V |