Summary of Study ST001368

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000935. The data can be accessed directly via it's Project DOI: 10.21228/M8TT3R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001368
Study TitleMalnutrition and Liver Metabolomics Intervention (part-II)
Study SummaryRP-UPLC-FTMS (+/- ion detection) and HILIC-FTMS (+/- ion detection) were conducted on murine livers from healthy (CON) and malnourished model (MBG) mice. To examine the impact of dietary intervention the same untargeted metabolomics was conducted following an intervention treatment (CON, MBG, C-MBG, MBG-R groups). C-MBG = healthy to malnourished, MBG-R = malnourished model reversed on a healthy diet.
Institute
University of British Columbia
DepartmentMicrobiology and Immunology
LaboratoryB. Brett Finlay
Last NameBauer
First NameKylynda
AddressMichael Smith Laboratories, #301 – 2185 East Mall, University of British Columbia Vancouver, British Columbia Canada, V6T 1Z4
Emailkcbauer@msl.ubc.ca
Phone(604) 822-2210
Submit Date2020-04-28
Num Groups4
Total Subjects32
Num Females32
Analysis Type DetailLC-MS
Release Date2020-08-03
Release Version1
Kylynda Bauer Kylynda Bauer
https://dx.doi.org/10.21228/M8TT3R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN002279 AN002280 AN002281 AN002282
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters BEH C8 (50 x 2.1mm,1.7um) Waters BEH C8 (50 x 2.1mm,1.7um) Waters HILIC (100 x 2.1mm,1.8um) Waters HILIC (100 x 2.1mm,1.8um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo LTQ-Orbitrap Velos Pro Thermo LTQ-Orbitrap Velos Pro Thermo LTQ-Orbitrap Velos Pro Thermo LTQ-Orbitrap Velos Pro
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Abundance Abundance Abundance abundance

MS:

MS ID:MS002123
Analysis ID:AN002279
Instrument Name:Thermo LTQ-Orbitrap Velos Pro
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods).
Ion Mode:POSITIVE
  
MS ID:MS002124
Analysis ID:AN002280
Instrument Name:Thermo LTQ-Orbitrap Velos Pro
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods).
Ion Mode:NEGATIVE
  
MS ID:MS002125
Analysis ID:AN002281
Instrument Name:Thermo LTQ-Orbitrap Velos Pro
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods).
Ion Mode:POSITIVE
  
MS ID:MS002126
Analysis ID:AN002282
Instrument Name:Thermo LTQ-Orbitrap Velos Pro
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods).
Ion Mode:NEGATIVE
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