Summary of Study ST001943
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001153. The data can be accessed directly via it's Project DOI: 10.21228/M8P10F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001943 |
| Study Title | Urinary signature of chronic kidney disease in patients with severe obesity by CE-MS |
| Study Type | Human nephropathy in CKD obese patients |
| Study Summary | Urine metabolomic characterization of severe obese patients with and without chronic kidney disease (CKD) by CE-MS. Analysis was performed in patients before and after bariatric surgery. In the present studio, samples obtained from CKD patients with severe obesity before bariatric surgery will be referred as OD (obese disease). Samples obtained from CKD patients with severe obesity after bariatric surgery will be referred as ODBS (Obese disease bariatric surgery). Patients with severe obesity without CKD will be referenced as O (obese) and after BS, OBS (obese bariatric surgery). In healthy group, when results refer to the first void urine samples, the acronym will be Healthy1V, and the acronym for urine samples collected at 24-hour will be Healthy24h. |
| Institute | University Rey Juan Carlos |
| Department | Basics Science of Health |
| Laboratory | LAFEMEX |
| Last Name | Lanzon |
| First Name | Borja |
| Address | Avenida de Atenas S/N |
| borja.lanzon@urjc.es | |
| Phone | 663692554 |
| Submit Date | 2021-08-19 |
| Num Groups | 6 |
| Total Subjects | 27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | CE-MS |
| Release Date | 2021-11-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003187 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | EI |
| MS instrument type | CE-TOF-MS |
| MS instrument name | Agilent 6224 TOF |
| Ion Mode | POSITIVE |
| Units | Area |
MS:
| MS ID: | MS002965 |
| Analysis ID: | AN003187 |
| Instrument Name: | Agilent 6224 TOF |
| Instrument Type: | CE-TOF-MS |
| MS Type: | EI |
| MS Comments: | Ions in gaseous state were directed to the TOF using two different voltages for the fragmentor (125 V and 200 V) and voltages of 65 V and 750 V for the skimmer and the octopole, respectively. Mass range was set from 70 to 1050 Da at a scanning rate of 1.02 scans per second. Data was collected in ESI positive ion mode. MassHunter Workstation version B.06.01 (Agilent Technologies) was used to monitor CE-MS analysis. Voltage of 125 V in the fragmentor was applied to ordinary samples and QC control samples. Data acquired at 125 V, equivalent to MS1, provided information about adducts, isotopes, multimers and fragments ions with low intensity. Voltage of 200 V in the fragmentor was applied to four QC (covering the four types of QC prepared). Data acquired at 200 V is similar to data obtained in 10 eV MS/MS, although has been previously reported that some fragment ions could differ. An equilibration QC was prepared to adapt and prepare the equipment for the conditions of the samples to be analysed. Equilibration QC was prepared as a pool of the four types of QC used to cover completely the biological diversity of the samples. Equilibration QC was analysed before samples. QC samples were disposed throughout the instrumental analysis. |
| Ion Mode: | POSITIVE |
