Summary of Study ST002430
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001563. The data can be accessed directly via it's Project DOI: 10.21228/M8NX4M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002430 |
| Study Title | Insights from a Multi-Omics Integration (MOI) Study in Oil Palm (Elaeis guineensis Jacq.) Response to Abiotic Stresses: Part Two—Drought |
| Study Type | Multi-Omics Integration (MOI) Study |
| Study Summary | Drought and salinity are two of the most severe abiotic stresses affecting agriculture Worldwide and bear some similarities in the response of plants to them. The first is also known as osmotic stress and shows similarities mainly with the osmotic effect, the first phase of salinity stress. Multi-Omics Integration (MOI) offers a new opportunity for the non-trivial challenge of unraveling the mechanisms behind multigenic traits, such as drought and salinity resistance. The current study carried out a comprehensive, large-scale, single-omics analysis (SOA) and MOI studies on the leaves of young oil palm plants submitted to water deprivation. After performing SOA, 1,955 DE enzymes from transcriptomics analysis, 131 DE enzymes from proteomics analysis, and 269 DE metabolites underwent MOI analysis, revealing several pathways affected by this stress, with at least one DE molecule in all three omics platforms used. Besides, the similarities and dissimilarities in the molecular response of those plants to those two abiotic stresses underwent mapping. Cysteine and methionine metabolism (map00270) was the most affected pathway in all scenarios evaluated. The correlation analysis revealed that 91.55% of those enzymes expressed under both stresses had similar qualitative profiles, corroborating the already known fact that plant responses to drought and salinity show several similarities. At last, the results shed light on some candidate genes for engineering crop species resilient to both abiotic stresses. |
| Institute | The Brazilian Agricultural Research Corporation (Embrapa) |
| Department | Embrapa Agroenergy |
| Laboratory | Genetics and Plant Biotechnology |
| Last Name | Souza Jr |
| First Name | Manoel Teixeira |
| Address | Parque Estacao Biologica, Final Avenida W3 Norte - Asa Norte, Brasilia, Distrito Federal, 70770901, Brazil |
| manoel.souza@embrapa.br | |
| Phone | +55.61.3448.3210 |
| Submit Date | 2022-09-28 |
| Publications | https://doi.org/10.1038/s41598-021-97835-x |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-01-20 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003955 | AN003956 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | Waters Acquity BEH C18 (150 x 2mm, 1.7um) | Waters Acquity BEH C18 (150 x 2mm, 1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker maXis Impact qTOF | Bruker maXis Impact qTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak intensity | Peak intensity |
MS:
| MS ID: | MS003690 |
| Analysis ID: | AN003955 |
| Instrument Name: | Bruker maXis Impact qTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | High-resolution mass spectrometry (HRMS) was performed in a MaXis 4G Q-TOF MS system (Bruker Daltonics, Germany) using an electrospray source in the positive and negative ion modes (ESI(+)–MS and ESI(−)–MS). The MS instrument settings used were: endplate offset, 500 V; capillary voltage, 3800 V; nebulizer pressure, 4 bar; dry gas flow, 9 L/min, dry temperature, 200 °C; and column temperature, 40 °C. The acquisition spectra rate was 3.00 Hz, monitoring a mass range from 70 to 1200 m/z. Sodium formate solution (10 mM NaOH solution in 50/50 v/v isopropanol/water containing 0.2% formic acid) was directly injected through a 6-port valve at the beginning of each chromatographic run to external calibration. UHPLC–MS data was acquired by the HyStar Application version 3.2 (Bruker Daltonics, Germany), and data processing was done using Data Analysis 4.2 (Bruker Daltonics, Germany). |
| Ion Mode: | POSITIVE |
| MS ID: | MS003691 |
| Analysis ID: | AN003956 |
| Instrument Name: | Bruker maXis Impact qTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | High-resolution mass spectrometry (HRMS) was performed in a MaXis 4G Q-TOF MS system (Bruker Daltonics, Germany) using an electrospray source in the positive and negative ion modes (ESI(+)–MS and ESI(−)–MS). The MS instrument settings used were: endplate offset, 500 V; capillary voltage, 3800 V; nebulizer pressure, 4 bar; dry gas flow, 9 L/min, dry temperature, 200 °C; and column temperature, 40 °C. The acquisition spectra rate was 3.00 Hz, monitoring a mass range from 70 to 1200 m/z. Sodium formate solution (10 mM NaOH solution in 50/50 v/v isopropanol/water containing 0.2% formic acid) was directly injected through a 6-port valve at the beginning of each chromatographic run to external calibration. UHPLC–MS data was acquired by the HyStar Application version 3.2 (Bruker Daltonics, Germany), and data processing was done using Data Analysis 4.2 (Bruker Daltonics, Germany). |
| Ion Mode: | NEGATIVE |
