Summary of Study ST002699

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001671. The data can be accessed directly via it's Project DOI: 10.21228/M8Q716 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002699
Study TitleSpatially resolved metabolomics and isotope tracing reveal dynamic metabolic responses of dentate granule neurons with acute stimulation
Study SummaryKCl timecourse|Neuronal activity creates an intense energy demand that must be met by rapid metabolic responses. To define the metabolic adaptations of the neuron-enriched dentate granule cell (DGC) layer within the native tissue environment, we utilize acute hippocampal brain slices and perform fast metabolite preservation followed by mass spectrometry imaging (MALDI-MSI) to obtain spatially resolved metabolomics and isotope tracing data. We find that membrane depolarization promotes broad metabolic changes, including increased glycolytic activity in DGCs which is accompanied by mobilization of endogenous inosine into pentose phosphates.
Institute
Harvard University
Last NameYellen
First NameGary
Address220 Longwood Ave
Emailgary_yellen@hms.harvard.edu
Phone617-432-0137
Submit Date2023-04-04
Raw Data AvailableYes
Raw Data File Type(s)imzML
Analysis Type DetailMALDI-MS
Release Date2023-08-08
Release Version1
Gary Yellen Gary Yellen
https://dx.doi.org/10.21228/M8Q716
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004374
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type MALDI
MS instrument type QTOF
MS instrument name Bruker timsTOF fleX
Ion Mode NEGATIVE
Units a.u.

MS:

MS ID:MS004123
Analysis ID:AN004374
Instrument Name:Bruker timsTOF fleX
Instrument Type:QTOF
MS Type:MALDI
MS Comments:Mass spectra were acquired on a timsTOF fleX in negative ion mode. Hippocampal sections were outlined as individual ROIs with a pixel size of 20 ┬Ám. Most metabolites were acquired in a single imaging run (m/z range 50 - 1000). Each MSI pixel consisted of 1000 laser shots. The laser Smartbeam parameter was set to single with a frequency of 10 kHz.
Ion Mode:NEGATIVE
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