Summary of Study ST002737
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001702. The data can be accessed directly via it's Project DOI: 10.21228/M8Q42J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002737 |
Study Title | 2’-fucosyllactose prevents colitis |
Study Type | untargeted metabolomics analysis |
Study Summary | Human milk-derived 2’-fucosyllactose (2’-FL) consumption is associated with health benefits in infancy that extend into adulthood. However, the exact biological functions of 2’-FL and corresponding mechanisms of action remain largely unknown. Here, we investigated the impact of 2’-FL on gut microbial metabolism for the prevention of colitis in adulthood. The gut microbiota from adult mice treated with 2’-FL showed an increase in abundance of several health-associated genera, including Bifidobacterium, and exhibited preventive effects on colitis. Microbial metabolic analysis demonstrated that 26 pathways that are significantly different between non-inflammatory bowel disease individuals and patients with ulcerative colitis (UC) are significantly regulated by 2’-FL in mice, indicating that 2’-FL has the potential to directly regulate dysregulated microbial metabolism in UC. Exploratory metabolomics of Bifidobacterium infantis identified novel secreted metabolites significantly enriched by 2’-FL consumption, including pantothenol. Remarkably, pantothenate significantly protects mucosal barrier and mitigates colitis in adult mice. Thus 2’-FL-modulated gut microbial metabolism may contribute to the prevention of intestinal inflammation in adulthood. |
Institute | Vanderbilt University |
Department | Chemistry |
Laboratory | Center for Innovative Technology |
Last Name | CODREANU |
First Name | SIMONA |
Address | 1234 STEVENSON CENTER LANE |
SIMONA.CODREANU@VANDERBILT.EDU | |
Phone | 6158758422 |
Submit Date | 2023-06-15 |
Num Groups | 2 |
Total Subjects | 10 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-12-18 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004439 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Vanquish UHPLC binary system |
Column | Thermo Hypersil Gold (100 x 2.1mm, 1.9um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | time_m/z |
MS:
MS ID: | MS004186 |
Analysis ID: | AN004439 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The acquired RPLC-HRMS raw data from five (5) biological replicates from each sample type were imported, processed, normalized and reviewed using Progenesis QI v.3.0 (Non-linear Dynamics, Newcastle, UK). All MS and MS/MS sample runs were aligned against a pooled QC reference run. Unique ions (retention time and m/z pairs) were de-adducted and de-isotoped to generate unique “features” (or retention time and m/z pairs). Data were normalized to all features and cleaned by removing spectral features >25% CV in the pooled QC samples. Sample process and instrument variability were also assessed to determine sample acceptance. Briefly, QA metrics for sample process variability and instrument variability are ≤10% CV and ≤5% CV, respectively. In these studies, no samples were identified as outliers. Statistical analyses were performed in Progensis QI using variance stabilized measurements achieved through log normalization to calculate p-values by one-way analysis of variance (ANOVA) test. Significantly changed metabolites were chosen with the criteria p-value <0.05 and |FC| > 2. |
Ion Mode: | POSITIVE |