Summary of Study ST002919
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001814. The data can be accessed directly via it's Project DOI: 10.21228/M87D9M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002919 |
| Study Title | Short-term metabolic insulin response of DINCH- and MINCH-treated cells |
| Study Summary | In the second part of the project, we examined the short-term metabolic insulin response of DINAH- and MINCH-treated cells and compared them with rosiglitazone-differentiated cells. For this purpose, the human SGBS preadipocyte cell line was exposed to differentiation media conditioned with DINCH (10 nM or 10 µM), MINCH (10 nM or 10 µM), or rosiglitazone, and the insulin response was measured by analyzing the changes in glycolysis and PPP between insulin-stimulated and non-insulin-stimulated cells. In conclusion, the insulin response of glycolysis and PPP of cells treated with 10 µM MINCH, but not with 10 nM MINCH or DINCH, was similar to cells differentiated with rosiglitazone. |
| Institute | Helmholtz Centre for Environmental Research |
| Last Name | Engelmann |
| First Name | Beatrice |
| Address | Permoserstr. 15 |
| beatrice.engelmann@ufz.de | |
| Phone | 00493412351099 |
| Submit Date | 2023-10-08 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-11-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004789 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Waters Xselect XP HSS T3 (150 x 2.1mm, 2.5µm) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 6500+ QTrap |
| Ion Mode | NEGATIVE |
| Units | Peak AUC |
MS:
| MS ID: | MS004535 |
| Analysis ID: | AN004789 |
| Instrument Name: | ABI Sciex 6500+ QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | For identification and quantitation, a scheduled MRM method was used, with specific transitions for every metabolite. Data acquisition and peak integration were performed in Analyst® software (Version 1.7.1). |
| Ion Mode: | NEGATIVE |
