Summary of Study ST002188
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001395. The data can be accessed directly via it's Project DOI: 10.21228/M8DB02 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST002188 |
Study Title | Metabolomic profiling reveals muscle metabolic changes following iliac arteriovenous fistula creation in mice (Lipid) |
Study Type | Study of the skeletal muscle metabolome in mice with iliac arteriovenous fistula via 1H NMR |
Study Summary | In the present study, we hypothesize that the creation of an iliac AVF would result in significant alterations to the limb muscle metabolome. Recently, our group developed a new murine model to address the pathophysiology of access-related hand dysfunction (ARHD) in mice, where AVF creation is performed in the iliac artery/vein. Because of the anatomical location of the AVF creation, this model produces clinically relevant changes in the mouse hindlimb including hemodynamic alterations, muscle weakness, and mitochondrial function impairment. |
Institute | University of Florida |
Department | Applied Physiology and Kinesiology |
Laboratory | Rm 42 and Rm 43 |
Last Name | Ryan |
First Name | Terence |
Address | 1864 Stadium Rd, FLG 114, Gainesville, FL, 32611, USA |
ryant@ufl.edu | |
Phone | 352-294-1700 |
Submit Date | 2022-02-17 |
Num Groups | 4 |
Total Subjects | 34 |
Num Males | All |
Study Comments | Metabolomic study via NMR |
Publications | Frontiers |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2022-08-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000240 |
Analysis ID: | AN003582 |
Instrument Name: | Bruker Avance III Cryo800 MHz 54 mm |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Field Frequency Lock: | Deuterated chloroform |
Standard Concentration: | 10mM pyrazine |
Spectrometer Frequency: | 799.9 |
NMR Probe: | CP TXI CryoProbe |
NMR Solvent: | Deuterated chloroform |
NMR Tube Size: | 1.5 mm O.D. |
Shimming Method: | Topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | none |
Pulse Width: | 90-degree |
Receiver Gain: | 228 |
Offset Frequency: | None |
Chemical Shift Ref Cpd: | CDCl3 at 7.26 ppm and pyrazine at 8.61 ppm |
Temperature: | 25 o C |
Number Of Scans: | 128 scans |
Dummy Scans: | 8 |
Acquisition Time: | 4 s |
Relaxation Delay: | 1 s |
Spectral Width: | 9523.8 |
Num Data Points Acquired: | 38094 |
Real Data Points: | 65536 |
Line Broadening: | 0.22 Hz |
Zero Filling: | 65,536 points |
Apodization: | Exponential |
Baseline Correction Method: | Spline |
Chemical Shift Ref Std: | 7.26ppm for CDCl3 |
Binned Increment: | None |
Binned Data Excluded Range: | None |