Summary of Study ST002463
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001590. The data can be accessed directly via it's Project DOI: 10.21228/M85Q7B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002463 |
| Study Title | Tryptophan metabolites in mouse serum |
| Study Summary | Analyzing murine sera to investigate into which tryptophan metabolites in the host circulation are derived from diet, host metabolism and/or the gut microbiome. Blood was collected from the portal vein of conventional C567BL/6J mice and sera analyzed by targeted LC-MS. To test this hypothesis and evaluate the necessity of microorganisms to produce these examined tryptophan metabolites, sera were collected from germ-free (GF) mice and subjected to the same targeted metabolomic analysis. Having generated Trp metabolite profiles from sera, we asked if altering the diet would significantly change circulating concentrations. One of two groups of conventional C57BL/6J mice with ad libitum access to a standard laboratory chow were switched onto a semi-purified diet (AIN-93G) while the second remained on standard chow. After one week, portal vein blood was collected and analyzed using targeted LC-MS. |
| Institute | Pennsylvania State University |
| Last Name | DONG |
| First Name | FANGCONG |
| Address | 309 Life Sciences Building, University Park, PA 16802 |
| fxd93@psu.edu | |
| Phone | 8146990203 |
| Submit Date | 2023-01-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-06 |
| Release Version | 1 |
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Project:
| Project ID: | PR001590 |
| Project DOI: | doi: 10.21228/M85Q7B |
| Project Title: | Analyzing murine sera to investigate into which tryptophan metabolites in the host circulation are derived from diet, host metabolism and/or the gut microbiome. |
| Project Type: | MS quantitative study |
| Project Summary: | Analyzing murine sera to investigate into which tryptophan metabolites in the host circulation are derived from diet, host metabolism and/or the gut microbiome. Blood was collected from the portal vein of conventional C567BL/6J mice and sera analyzed by targeted LC-MS. To test this hypothesis and evaluate the necessity of microorganisms to produce these examined tryptophan metabolites, sera were collected from germ-free (GF) mice and subjected to the same targeted metabolomic analysis. Having generated Trp metabolite profiles from sera, we asked if altering the diet would significantly change circulating concentrations. One of two groups of conventional C57BL/6J mice with ad libitum access to a standard laboratory chow were switched onto a semi-purified diet (AIN-93G) while the second remained on standard chow. After one week, portal vein blood was collected and analyzed using targeted LC-MS. |
| Institute: | The Pennsylvania State University |
| Last Name: | DONG |
| First Name: | FANGCONG |
| Address: | 309 Life Sciences Building, State college, PA, 16802, USA |
| Email: | fxd93@psu.edu |
| Phone: | 8146990203 |
