Summary of Study ST000069
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000066. The data can be accessed directly via it's Project DOI: 10.21228/M8QG6H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000069 |
| Study Title | Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice (via blood and tissue) |
| Study Summary | Lung samples were obtained at 15 weeks from 56 mice that had received either chow, a low fat diet, or a high fat diet and that were uninfected, 4 days post-infection, or 8 days post-infection. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-06-14 |
| Num Groups | 9 |
| Total Subjects | 55 |
| Raw Data Available | No |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-06-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000084 |
| Sampleprep Summary: | Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down and reconstituted in 100uL of 95:5 water:methanol. |
| Sampleprep Protocol Filename: | RTI__BECK-LUNG_DIET_Metabolomics_Procedure.docx |
| Processing Method: | Homogenization |
| Extraction Method: | Acetonitrile |
| Extract Storage: | -80 C |
| Sample Resuspension: | 95:5 Water:Methanol |
| Sample Spiking: | L-Tryptophan-d5 |
| Organ: | Lung |
