Summary of Study ST000069

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000066. The data can be accessed directly via it's Project DOI: 10.21228/M8QG6H This work is supported by NIH grant, U2C- DK119886.


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Study IDST000069
Study TitleMetabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice (via blood and tissue)
Study SummaryLung samples were obtained at 15 weeks from 56 mice that had received either chow, a low fat diet, or a high fat diet and that were uninfected, 4 days post-infection, or 8 days post-infection.
University of North Carolina
DepartmentSystems and Translational Sciences
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Submit Date2014-06-14
Num Groups9
Total Subjects55
Raw Data AvailableNo
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2015-06-01
Release Version1
Susan Sumner Susan Sumner application/zip

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Sample Preparation:

Sampleprep ID:SP000084
Sampleprep Summary:Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down and reconstituted in 100uL of 95:5 water:methanol.
Sampleprep Protocol Filename:RTI__BECK-LUNG_DIET_Metabolomics_Procedure.docx
Processing Method:Homogenization
Extraction Method:Acetonitrile
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5