Summary of Study ST000220
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000050. The data can be accessed directly via it's Project DOI: 10.21228/M8RG6T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000220 |
| Study Title | Small cell lung cancer metabolome (part II) |
| Study Type | Comparison of normal, lung adenocarcinoma and SCLC tissue metabolomes |
| Study Summary | In addition to the generation and analysis of metabolomics data on cell lines, samples of normal lung tissue, adenocarcinoma lung tissue and small cell lung carcinoma tissue (seven samples/group) were processed and evaluated metabolite profile differences under the scope of the pilot and feasibility study. These data can be correlated to the metabolite profiles defined in the SCLC and NSCLC cell lines and integrated with the ABPP-determined metabolic kinases to identify distinct metabolic signatures or biomarkers (?oncometabolites?) that distinguish small cell lung cancer from non-small cell lung cancer. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2015-06-26 |
| Num Groups | 3 |
| Total Subjects | 21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Uploaded File Size | 58 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-07-08 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000241 |
| Sampleprep Summary: | Pulverized tissue samples were incubated in 50:50 Acetonitrile:Water for 1 hr, vortexed and centrifuged. Supernatants were transferred to new tubes along with isotopocially labled internal standard and samples were lyophilized to dryness. Dried samples were resuspended in 95:5 Water:Methanol and an aliquot was transferred to autosampler vials forbroad spectrum metabolomics data analysis acquisition. |
| Processing Method: | Pulverization of frozen tissue (performed at Moffitt and supplied to RTI) |
| Extraction Method: | 50:50 Acetonitrile:Water |
| Extract Storage: | -80 C |
| Sample Resuspension: | 95:5 Water:Methanol |
| Sample Spiking: | L-Tryptophan-d5 |
| Organ: | Lung |
