Summary of Study ST000364

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000289. The data can be accessed directly via it's Project DOI: 10.21228/M8PK67 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000364
Study Title	Metabolomics Profiling of NEST Cord Blood Plasma
Study Type	Metabolomics
Study Summary	This metabolomics pilot study evaluated cord blood plasma from these children to understand how these factors influence the metabotype.
Institute	University of North Carolina
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-03-09
Raw Data Available	Yes
Raw Data File Type(s)	1r
Analysis Type Detail	NMR
Release Date	2017-10-03
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000392
Sampleprep Summary:	An aliquot (125 μl) of thawed plasma from each study sample was mixed with 375 μl of methanol, vortexed, and centrifuged. 400 uL of the supernatant was dried in vacuum overnight. Each sample was reconstituted with 250 μL of 0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6. Two random study pools were created by mixing 30 μl of plasma from a subset of study samples. Five replicates were created of each pool for a total of ten pooled samples, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000392

Sampleprep Summary:

An aliquot (125 μl) of thawed plasma from each study sample was mixed with 375 μl of methanol, vortexed, and centrifuged. 400 uL of the supernatant was dried in vacuum overnight. Each sample was reconstituted with 250 μL of 0.2 mM phosphate buffer (pH 7.5) containing 10% Chenomx Internal Standard - 0.5 mM DSS-d6. Two random study pools were created by mixing 30 μl of plasma from a subset of study samples. Five replicates were created of each pool for a total of ten pooled samples, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.