Summary of Study ST000409

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000409
Study Title	Metabolomic analysis of oxytocin effects on social deficits in mice (part II)
Study Summary	The goal of this study is to determine the prosocial hormone oxytocin (OT) effects on metabolomic profiles in feces.
Institute	University of North Carolina
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-06-02
Raw Data Available	Yes
Raw Data File Type(s)	1r
Analysis Type Detail	NMR
Release Date	2018-06-05
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000437
Sampleprep Summary:	Fecal pellets were transferred into a pre-labeled MagnaLyser tube and massed. Phosphate buffer was added depending on the mass of each sample (500 uL for up to 100 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. The supernatant of each sample was transferred into pre-labeled 0.2 um nylon filter tube and centrifuged. A volume of each filtrate required to analyze 25 mg of sample was aliquoted, mixed with a volume of phosphate buffer to bring the total volume to 630 uL, and 70 uL of Chenomx ISTD. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume. Five replicates were created, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000437

Sampleprep Summary:

Fecal pellets were transferred into a pre-labeled MagnaLyser tube and massed. Phosphate buffer was added depending on the mass of each sample (500 uL for up to 100 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. The supernatant of each sample was transferred into pre-labeled 0.2 um nylon filter tube and centrifuged. A volume of each filtrate required to analyze 25 mg of sample was aliquoted, mixed with a volume of phosphate buffer to bring the total volume to 630 uL, and 70 uL of Chenomx ISTD. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume. Five replicates were created, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.