Summary of Study ST000414
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000323. The data can be accessed directly via it's Project DOI: 10.21228/M8ZS3N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000414 |
| Study Title | Metabolomics-based screening of the Malaria Box reveals both novel and established mechanisms of action |
| Study Type | Drug incubation |
| Study Summary | Measuring the impact of MMV Malaria Box compounds on metabolism in Plasmodium falciparum-infected red blood cells |
| Institute | Monash Institute of Pharmaceutical Sciences |
| Department | Drug delivery, disposition and dynamics |
| Laboratory | Creek lab |
| Last Name | Creek |
| First Name | Darren |
| Address | 381 Royal Parade, Parkville, Melbourne, VIC3052, Australia |
| Darren.Creek@monash.edu | |
| Phone | N/A |
| Submit Date | 2016-06-16 |
| Num Groups | 126 |
| Total Subjects | 18 |
| Study Comments | 524 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-07-10 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000442 |
| Sampleprep Summary: | Culture medium was removed by aspiration, and the metabolism of the settled iRBCs quenched by addition of ice-cold phosphate buffered saline (PBS). Subsequent steps were performed on ice. Cells were pelleted by centrifugation for 5 min at 1000 × g and the PBS supernatant removed prior to the addition of 135 µL methanol (containing internal standard compounds: CHAPS, CAPS and PIPES) and rapid mixing by pipetting three times to extract iRBC metabolites. Samples were left on ice with gentle agitation for 60 minutes, then centrifuged at 3000 × g to remove the insoluble material. Supernatants were transferred to glass HPLC vials and stored (< 4 months) at -80 °C until analysis. |
| Processing Method: | Lysis with organic solvent and mixing at 4°C |
| Processing Storage Conditions: | on ice or 4°C |
| Extraction Method: | Methanol (10:1) |
| Extract Storage: | -80°C |
| Sample Spiking: | internal standards (CHAPS, CAPS, PIPES all at 1 µM) mixed in extraction solvent |
| Cell Type: | Plasmodium falciparum-infected red blood cells (8% parasitaemia and 3% haematocrit |
