Summary of Study ST000453
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000350. The data can be accessed directly via it's Project DOI: 10.21228/M8T30C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000453 |
Study Title | Metabolic Adaptation of Staphylococcus aureus to Host Immunity |
Study Type | Metabolomics Analysis of Methicillin-Resistant Staphylococcus aureus (MRSA) to host immunity. |
Study Summary | This project is intended to study the metabolic adaptation of Methicillin-Resistant Staphylococcus aureus (MRSA) to host immunity. Because of the nature of the samples RTI RCMRC worked with Dr. Anthony R. Richardson so that the samples would be extracted at the University of North Carolina at Chapel Hill under the condition that were optimized by RTI RCMRC for broad spectrum metabolomics analysis. |
Institute | University of North Carolina |
Department | RCMRC |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2017-09-10 |
Num Groups | 29 |
Total Subjects | 29 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000481 |
Sampleprep Summary: | Methylillin-resistant S. aureus (MRSA) bacterial culture extracts re-suspended in water were provided to RTI RCMRC for analysis. These extracts were stored at -80 °C upon arrival. For processing, samples were dried overnight on a lyophilizer. The residue was then reconstituted with 150 µL of an ice-cold solution of reconstitution buffer (0.005 mg/mL Tryptophan-d5 in 95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 2 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and 100 µL of the supernatants were transferred to autosampler vials for data acquisition. Whole study pooled QC samples were created by combining 40 µL aliquot from each of the reconstituted samples into a 2 mL LoBind eppendorf tube. The QC pooled sample was then vortexed for 15 sec and 100 µL of the total study pooled QC samples were transferred to five pre-labeled autosampler vials. |