Summary of Study ST000467
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000359. The data can be accessed directly via it's Project DOI: 10.21228/M8NC8M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000467 |
Study Title | Metabolomics of Saliva Samples Obtained from Subjects with Diabetes |
Study Summary | In this research, were are investigating the metabolic profile changes associated with well- and poorly-controlled type 1 and 2 diabetes and if there are distinct metabolite compounds that may be associated with glycemic control. The PI of the study collected whole unstimulated saliva samples were from subjects with well- and poorly-controlled type 1 and type 2 diabetes. Subjects were selected based on the level of A1C (<7= well-controlled and >7 = poorly controlled). Saliva samples were shipped to RTI RCMRC for a broad spectrum reverse phase metabolomics analysis. |
Institute | University of North Carolina |
Department | Discovery-Sciences-Technology (DST) |
Laboratory | Sumner Lab |
Last Name | Sumner |
First Name | Susan |
Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
susan_sumner @unc.edu | |
Phone | 704-250-5066 |
Submit Date | 2016-09-02 |
Num Groups | 4 |
Total Subjects | 40 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000495 |
Sampleprep Summary: | Study Samples and Whole Study Pooled QC Aliquots Preparation: Thawed aqueous saliva samples on ice. Samples were then vortexed and centrifuged at room temperature and at 16,000 rcf for four minutes. A 100 µL aliquot of each experimental sample was transferred to a labeled 2.0 mL Lo-Bind Eppendorf tube to make the study sample aliquots. Whole study pooled QC samples were created by combining 100 µL aliquot from each of the study samples into a 2 mL Lo-Bind eppendorf tube. This QC pooled sample was then vortexed for 30 seconds. Then, ten whole study pooled QC samples of 100 µL each were created. Sample Extraction To all samples (study and whole study pooled QC), 500 µL of Protein Precipitation Buffer with internal standard (0.0125 mg/mL of Tryptophan-d5 in methanol) was added to each tube and vortexed for 4 min at room temperature and at 5,000 rpm. The samples were then centrifuged at room temperature and at 16,000 rcf for 4 min. A 450 µL aliquot of the supernatant from each sample was transferred into pre-labeled 2.0 mL LoBind eppendorf tube and stored at -80 °C for one hour followed by a drying step on a lyophilizer. The residue was reconstituted in 100 µL of reconstitution buffer (95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 10 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and the supernatants were transferred to autosampler vials for data acquisition. |