Summary of Study ST000476

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000476
Study Title	Metabolomic analysis of oxytocin effects on social deficits in mice (part IV)
Study Summary	This metabolomics pilot study evaluated serum from mice treated with oxytocin and vehicle to understand how these factors influence the metabotype.
Institute	University of North Carolina
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-08-31
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2017-10-03
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000504
Sampleprep Summary:	30 µL of each serum sample was transferred to a new, pre-labeled 1.5 mL LoBind eppendorf tube to make the individual study samples, and 12 µL was transferred to a new, pre-labeled 1.5 mL LoBind Eppendorf to make the total pool. The total pool was aliquoted into 3 Total Pool QC Samples and 4 Column Equilibrium samples with volumes of 30 µL in 1.5 mL LoBind Eppendorf tubes. 600 uL of Lipid Extraction Solvent (PC(17:0/17:0) 50 µg/mL in 2:1 dichloromethane:methanol) was added to all samples, and samples were vortexed at 4,000 rpm for 2mins on multiple-tube vortexer. 120 µL of H2O was added to each tube and the samples were vortexed at 4,000 rpm for 1min. Samples sat at room temperature for 10 minutes, and were centrifuged at 16,000 rcf for 10 min at 10°C. 240 µL of the lower lipid-rich DCM layer was transferred into new pre-labeled 1.5 mL LoBind Eppendorf tubes, and rubber stoppers were inserted. Samples were placed at -80˚C for 60 mins, and then lyophilized for 19.5 hrs. 240 µL of ACN/IPA/H2O (65:30:5 v/v/v) was added to each sample, and the samples were vortexed at 4,000 rpm for 2mins and centrifuged at 16,000 rcf for 4 min. 225 µL of the supernatant was transferred to pre-labeled autosampler vials and 10 µL was injected into OrbiTrap Velos.

Sampleprep ID:

SP000504

Sampleprep Summary:

30 µL of each serum sample was transferred to a new, pre-labeled 1.5 mL LoBind eppendorf tube to make the individual study samples, and 12 µL was transferred to a new, pre-labeled 1.5 mL LoBind Eppendorf to make the total pool. The total pool was aliquoted into 3 Total Pool QC Samples and 4 Column Equilibrium samples with volumes of 30 µL in 1.5 mL LoBind Eppendorf tubes. 600 uL of Lipid Extraction Solvent (PC(17:0/17:0) 50 µg/mL in 2:1 dichloromethane:methanol) was added to all samples, and samples were vortexed at 4,000 rpm for 2mins on multiple-tube vortexer. 120 µL of H2O was added to each tube and the samples were vortexed at 4,000 rpm for 1min. Samples sat at room temperature for 10 minutes, and were centrifuged at 16,000 rcf for 10 min at 10°C. 240 µL of the lower lipid-rich DCM layer was transferred into new pre-labeled 1.5 mL LoBind Eppendorf tubes, and rubber stoppers were inserted. Samples were placed at -80˚C for 60 mins, and then lyophilized for 19.5 hrs. 240 µL of ACN/IPA/H2O (65:30:5 v/v/v) was added to each sample, and the samples were vortexed at 4,000 rpm for 2mins and centrifuged at 16,000 rcf for 4 min. 225 µL of the supernatant was transferred to pre-labeled autosampler vials and 10 µL was injected into OrbiTrap Velos.