Summary of Study ST000587

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000430. The data can be accessed directly via it's Project DOI: 10.21228/M8GC82 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000587
Study TitleMetabolomics of Exhaled Breath Condensate in Decompensated Heart Failure
Study SummaryThe aim of the study was to test weather characteristic differences or changes in metabolic profile exist between exhaled breath condensate (EBC) and saliva of healthy individuals and heart failure patients. EBC NMR profiling was performed.
Institute
Mayo Clinic
DepartmentCardiovascular Diseases
LaboratoryMayo Metabolomics Core
Last NameJohnson
First NameBruce
Address200 First Street SW, Rochester, MN 55905
Emailjohnson.bruce@mayo.edu
Phone507-284-4441
Submit Date2017-04-07
Analysis Type DetailNMR
Release Date2019-05-15
Release Version1
Bruce Johnson Bruce Johnson
https://dx.doi.org/10.21228/M8GC82
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000617
Sampleprep Summary:EBC: Transfer 800μl aliquots of EBC samples into a 1.5ml microcentrifuge tubes and dry down (lyophilize) each sample in a centrifugal vacuum evaporator for 4-6 h. Apply no heating during the drying process. In the dried EBC samples add 250μl of phosphate buffer and 250μl of 4mM TSP-d4 solution. Vortex for 20 s. Transfer the solution to 5mm NMR tube.
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