Summary of Study ST000615
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000447. The data can be accessed directly via it's Project DOI: 10.21228/M88P55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000615 |
Study Title | GC/MS measurement of sarcosine in urine samples |
Study Summary | GC/MS measurement of sarcosine in urine samples from prostate cancer and case control patients in a group of ancestry verified African American and European Americans. |
Institute | Baylor College of Medicine |
Last Name | Sreekumar |
First Name | Arun |
Address | One Baylor Plaza, Houston, TX, 77030, USA |
Arun.Sreekumar@bcm.edu | |
Phone | 713-798-3305 |
Submit Date | 2017-05-19 |
Analysis Type Detail | LC-MS |
Release Date | 2019-07-17 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000645 |
Sampleprep Summary: | Urine supernatants were diluted with methanol containing spiked labeled internal standards (d3-sarcosine and d3-alanine), and extracted overnight at 4°C, using 1:1 molar ratio of water/chloroform at room temperature, with constant shaking. The aqueous methanolic layer was recovered, dried and azeotroped twice by adding 100 mL dimethylformamide (DMF), mixed and dried using a speedvac for 30 minutes. Following this, 100 mL of DMF and N-methyl-N-tert-butylmethylsilyltrifluoroacetamide (MtBSTFA) + 1% t-butyl-dimethylchlorosilane were added and incubated at 60°C for 1 hr. The samples were resuspended in ethyl acetate and injected into a GC-MS. |