Summary of Study ST000882
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000611. The data can be accessed directly via it's Project DOI: 10.21228/M8XQ41 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000882 |
| Study Title | Untargeted metabolomics data of leprosy patients with type 1 reaction |
| Study Type | Untargeted MS qualitative analysis in retrospective serum samples of leprosy patients with type 1 reaction |
| Study Summary | Untargeted metabolomics-based analyses of sera from 7 patients with and 9 without T1R were conducted via liquid chromatography–mass spectrometry. |
| Institute | Colorado State University |
| Department | Department of Microbiology, Immunology, and Pathology |
| Laboratory | Belisle |
| Last Name | Silva |
| First Name | Carlos |
| Address | Rampart Road, 1682 Campus Delivery |
| cadriano@rams.colostate.edu | |
| Phone | 9702154962 |
| Submit Date | 2017-06-19 |
| Num Groups | 2 |
| Total Subjects | 16 |
| Num Males | 5 |
| Num Females | 11 |
| Study Comments | 2 groups (first group was leprosy patients with type 1 reaction, N=9; and second group was leprosy patients without type 1 reaction, N=7) 5 males (3 were leprosy patients with type 1 reaction and 2 were leprosy patients without type 1 reaction), 11 females (4 were leprosy patients with type 1 reaction and 7 were leprosy patients without type 1 reaction). For this study we used retrospective sera samples. |
| Publications | J Infect Dis. 2017 Feb 1;215(3):431-439. doi: 10.1093/infdis/jiw541. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-06-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000923 |
| Sampleprep Summary: | The retrospective serum samples (80 microliters[ul]) were incubated with 3 volumes of cold methanol (100%) for 1 hour at -20C. After this incubation, the samples were centrifuged for 30 minutes at 18,000 xg at 4C to clear the supernatant. The supernatant was then transferred and dried under vacuum. Samples were re-suspended in 80 μl of 50% methanol. Prior to the injections on the LC-MS instrument, the samples were centrifuged for 30 minutes at 18,000 xg at 4oC. |
| Sampleprep Protocol ID: | SP000100 |
| Sampleprep Protocol Filename: | 060613_Methanol_Serum_Extraction |
| Sampleprep Protocol Comments: | Use always nitrile gloves. Do not use gloves with powder. Use pipette tips with aerosol barrier. |
| Processing Storage Conditions: | -80C |
| Extraction Method: | Methanol extraction |
| Extract Cleanup: | Centrifugation |
| Extract Storage: | -80C |
| Sample Resuspension: | 50% methanol |
| Sample Derivatization: | no derivatization process was performed |
