Summary of Study ST000901

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000461. The data can be accessed directly via it's Project DOI: 10.21228/M8G609 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST000901
Study Title	Omega-6 and omega-3 oxylipins are implicated in soybean oil-induced obesity in mice - Plasma (part V)
Study Summary	Characterization and analysis of metabolomic, proteomic and metabolic profiles of C57/Bl6N mice fed various high fat diets
Institute	University of California, Davis
Last Name	Yang
First Name	Jun
Address	207 Everson Hall, Shields One Ave, Davis CA 95616
Email	junyang@ucdavis.edu
Phone	5307525109
Submit Date	2017-07-26
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2017-11-26
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000945
Sampleprep Summary:	Preparation of SPE on vacuum manifold: 1. Clean 60 mg Oasis HLB (Waters) spe column with 1 column volume ethyl acetate followed by 2 column volumes MeOH 2. Condition column with 2 column volume 0.1% acetic acid :5% MeOH solution 3. Close valves tightly when meniscus reaches frit over sorbent bed. Sample extraction by SPE on vacuum manifold: 4. If not in sample at collection, add 10 µL of 0.2 mg/mL of EDTA and BHT in MeOH: H2O (1:1) to sorbent bed of all columns 5. Vortex each sample a few seconds to homogenize just prior to loading. 6. Load 250µL plasma sample into SPE column. 7. Spike each column with 10µl 500nM surrogate solution 8. Load 1.5ml of 0.1% acetic acid: 5% MeOH solution to each column and let sit a few minutes. 9. Open valves to extract by gravity (use low vacuum only if necessary) 10. Wash SPE with 2 column volumes 0.1% acetic acid :5% MeOH solution 11. Pull aqueous plug from SPE with a few seconds high vacuum. 12. Further dry SPE with low vacuum. ~20min, -20psi. 13. For each sample, spike 2ml eppendorf tube with 6µl 30% glycerol in MeOH prior to eluate collection. 14. Elute spe into tube with 0.5 ml methanol followed by 1.5 mL of ethyl acetate 15. Evaporate extract until 2 µl glycerol plug is left, by Speed-vac. 16. Cap and freeze by liquid nitrogen (for shipping) or store at -80ºC until analysis.

Sampleprep ID:

SP000945

Sampleprep Summary:

Preparation of SPE on vacuum manifold: 1. Clean 60 mg Oasis HLB (Waters) spe column with 1 column volume ethyl acetate followed by 2 column volumes MeOH 2. Condition column with 2 column volume 0.1% acetic acid :5% MeOH solution 3. Close valves tightly when meniscus reaches frit over sorbent bed. Sample extraction by SPE on vacuum manifold: 4. If not in sample at collection, add 10 µL of 0.2 mg/mL of EDTA and BHT in MeOH: H2O (1:1) to sorbent bed of all columns 5. Vortex each sample a few seconds to homogenize just prior to loading. 6. Load 250µL plasma sample into SPE column. 7. Spike each column with 10µl 500nM surrogate solution 8. Load 1.5ml of 0.1% acetic acid: 5% MeOH solution to each column and let sit a few minutes. 9. Open valves to extract by gravity (use low vacuum only if necessary) 10. Wash SPE with 2 column volumes 0.1% acetic acid :5% MeOH solution 11. Pull aqueous plug from SPE with a few seconds high vacuum. 12. Further dry SPE with low vacuum. ~20min, -20psi. 13. For each sample, spike 2ml eppendorf tube with 6µl 30% glycerol in MeOH prior to eluate collection. 14. Elute spe into tube with 0.5 ml methanol followed by 1.5 mL of ethyl acetate 15. Evaporate extract until 2 µl glycerol plug is left, by Speed-vac. 16. Cap and freeze by liquid nitrogen (for shipping) or store at -80ºC until analysis.