Summary of Study ST001130
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000755. The data can be accessed directly via it's Project DOI: 10.21228/M8367J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001130 |
| Study Title | Urea cycle and 1C/serine metabolism in the prevention of oxygen induced retinopathy |
| Study Summary | Untargeted metabolite profiling links the urea cycle and 1C/serine metabolism in the prevention of oxygen induced retinopathy by hepatic HIF stabilization. Premature infants require oxygen supplementation to survive that is simultaneously toxic to developing tissues. We have demonstrated that hypoxia inducible factor (HIF) stabilization during hyperoxia prevents oxygen induced retinopathy (OIR) and lung disease. Here, untargeted metabolite profiling coupled to XCMS systems biology analysis finds that serine/1C and urea cycles dominate pathway enrichment graphs. MS1 peak areas and MS2 library matches reveal 50% or more increased levels of plasma and retina serine, glycine, hypotaurine, methionine, and taurine. In addition, N-acetylglutamate increased 4-fold in serum, while orotate, citrulline, arginine, aspartate, glutamine were at least 50% increased after HIF stabilization. Targeted data analysis in vivo finds that retinal serine and glycine were derived from liver. HIF-1α2lox/2lox; albumin-cre KO had reduced levels of serine and retinal glycine. Inhibition of 1C metabolism blocked rescue by HIF stabilization. The metabolic phenotype of mice protected from OIR by HIF stabilization is dependent on hepatic serine/1C metabolism and urea cycle. |
| Institute | Cole Eye Institute |
| Department | Cleveland Clinic |
| Last Name | Singh |
| First Name | Charandeep |
| Address | 9500 Euclid Avenue |
| cxs065@gmail.com | |
| Phone | (216) 444-8232 |
| Submit Date | 2019-01-31 |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-06-20 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001199 |
| Sampleprep Summary: | Metabolites from plasma were extracted by adding 20 µl of plasma to 500 µl of 50% cold acetonitrile. Samples were centrifuged at 15,000 x g for 10 min and 400 µl supernatant was carefully removed into fresh tubes without disturbing the cell pellet. Supernatants were injected directly into the LC-MS |
