Summary of Study ST001216
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000816. The data can be accessed directly via it's Project DOI: 10.21228/M86Q4H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001216 |
| Study Title | LCMS untargeted BAL Fluid analysis from COPD subjects |
| Study Summary | We performed untargeted liquid chromatography mass spectrometry (LCMS) on BAL and matched plasma from 115 subjects from the SPIROMICS cohort. |
| Institute | University of Colorado Anschutz Medical Campus; National Jewish Health |
| Last Name | Reisdorph |
| First Name | Nichole |
| Address | 12850 E Montview Blvd, Aurora, CO 80045 |
| Nichole.Reisdorph@cuanschutz.edu | |
| Phone | 303-724-9234 |
| Submit Date | 2019-07-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-07-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001291 |
| Sampleprep Summary: | 140uL BAL was aliquoted into a microcentrifuge tube containing 20 uL of internal standards. Samples were vortexed followed by protein precipitation with 560 uL cold methanol, and centrifugation (0 C for 15 mins at 18,000 x g). The supernatant was removed and placed into two autosampler vials (165 uL for Hydrophilic Interaction Liquid Chromatography (HILIC) and 495 uL for C18 analysis). The samples were dried in a centrifugal evaporator at 45 C for 2 hours. The samples for Hydrophilic Interaction Liquid Chromatography HILIC analysis were reconstituted in 30 uL of 95:5 water:acetonitrile. The samples for Reversed phase C18 analysis were reconstituted in 90 uL methanol. Samples were stored at -80C prior to MS analysis. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
