Summary of Study ST001238
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000829. The data can be accessed directly via it's Project DOI: 10.21228/M8HX2M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001238 |
Study Title | P falciparum asexual metabolomics following drug treatment (part-I) |
Study Summary | P falciparum infected human red blood cells were treated with 10X IC50 drug for 2.5 hours, followed by extraction and analysis of polar metabolites using HPLC-MS or HPLC-MS/MS |
Institute | Pennsylvania State University |
Last Name | Llinás |
First Name | Manuel |
Address | W126 Millennium Science Complex, University Park, PENNSYLVANIA, 16802, USA |
mul27@psu.edu | |
Phone | (814) 867-3527 |
Submit Date | 2019-08-08 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2019-09-23 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001314 |
Sampleprep Summary: | Following treatment parasites were pelleted, washed with 1 mL of ice-cold 1X PBS, and extracted using 1 mL of ice-cold 9:1 MeOH:Water, containing the internal standard 13C4,15N1-Aspartate. Supernatants were clarified before drying under nitrogen, followed by resuspension in HPLC grade water containing 1 µM chlorpropamide to 1x10^6 parasites/µL for HPLC-MS analysis. 10 µL was injected on a Thermo Exactive Plus Orbitrap mass spectrometer for HPLC-MS-based targeted metabolomics (modified from Lu W et al 2010). |