Summary of Study ST001247
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000834. The data can be accessed directly via it's Project DOI: 10.21228/M8W67P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001247 |
Study Title | Longitudinal Characterization of the Fecal Metabolome in Dogs with Idiopathic Inflammatory Bowel Disease |
Study Summary | Thirteen dogs diagnosed with idiopathic IBD, that previously failed to respond to treatment with elimination diets and metronidazole, were enrolled. Stool samples were collected from all dogs before initiating therapy with prednisone, after 3 and 8 weeks, and more than one year after beginning treatment. Thirteen healthy dogs were enrolled in the study as a control group. |
Institute | Texas A&M University |
Department | Department of Small Animal Clinical Sciences |
Laboratory | Gastrointestinal Laboratory |
Last Name | Pilla |
First Name | Rachel |
Address | 4474 TAMU |
rpilla@cvm.tamu.edu | |
Phone | 9798622861 |
Submit Date | 2019-09-03 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | MALDI-MS |
Release Date | 2020-06-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001323 |
Sampleprep Summary: | Fecal samples were lyophilized and approximately 10 mg was sent to the West Coast Metabolomics Center (WCMC) at University of California at Davis (http://metabolomics.ucdavis.edu/). Samples were analyzed on a gas chromatography time-of-flight mass spectrometry (GC–TOF–MS) platform, in accordance with published methods. Briefly, samples underwent homogenization and extraction, followed by centrifugation. Dried supernatant was resuspended in methanol/chloroform and internal standards were added, followed by drying and derivatization by methoxyamine hydrochloride and N-methyl-N-trimethylsilyltrifluoroacetamide. A volume of 0.5 μL was injected in splitless mode onto a Restek rtx5SilMS column on a temperature-gradient programmed GC (oven 50–330 °C at 20 °C/min, injector 50–250 °C at 12 °C/ sec) coupled with a Leco Pegasus IV mass spectrometer (scanning 70 spectra/sec from 80 to 500 Da, −70 eV ionization energy, 1800 V detector voltage) with helium carrier gas (1 mL/min). Raw data files were processed using ChromaTOF v. 2.32. BinBase algorithm matched spectra to database compounds, and quantification was reported by peak height of an ion at the specific retention index characteristic of the compound across all samples. Peak heights were normalized by average total peak-sums for identified compounds across each sample group. |