Summary of Study ST001291

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000873. The data can be accessed directly via it's Project DOI: 10.21228/M8V393 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001291
Study TitleMetabolomic study of disease progression in scrapie prion infected (RML) mice
Study TypeTimecourse experiment
Study SummaryWe used NMR spectroscopy to investigate metabolic perturbations in the brains of RML infected mice along the progression of prion disease at 30, 60, 90, 120, and 125 days post-inoculation.
University of Alberta
Last NameFu
First NameZelin
Address116 St & 85 Ave, Edmonton, AB T6G 2R3
Submit Date2019-12-16
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailNMR
Release Date2020-01-13
Release Version1
Zelin Fu Zelin Fu application/zip

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Sample Preparation:

Sampleprep ID:SP001372
Sampleprep Summary:Half brains were homogenized with Dounce tissue homogenizers to make 10% (w/v) homogenate. The homogenate is then subject to ultracentrifuge at 100,000xg for 1h at 4°C, the resulting supernatant was neutralized with Tris buffer (0.5M, pH6.8) one tenth the volume of the supernatant. Then the neutralized supernatant is passed through 3kDa molecular weight cut-off filters at 4000 rpm for 45 mins at 4°C. Filtered supernatant was tried at SpeedVac Vacuum Concentrator at low heating until try pellets were received. Try pellets were then dissolved with 530ul of water and 60 ul of D2O containing 5mM of DSS. A final volume of 600ul for each sample was achieved.