Summary of study ST001619

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001038. The data can be accessed directly via it's Project DOI: 10.21228/M8HX30 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001619
Study TitleMetabolomics Analysis: Opioid Addiction Project (Golestan Cohort Study) - NMR (part-II)
Study TypeUntargeted NMR Metabolomics Analysis
Study SummaryDrug addiction is a major threat to the public health in the US and many other countries. Opioid abuse is associated with increased risks for cancer, psychological complications, heart and lung disease, and infections of the liver and blood. Because metabolites are intrinsically involved in multiple metabolic pathways in vivo, the relative quantification of metabolites in body fluids (for example urine) can provide a profile of the metabolic state of an organism. Metabolomics is a powerful technique for revealing the impact of exposure on the overall biochemistry of an individual or system. Opioids can modify the output of urinary metabolites through many integrated neural and hormonal mechanisms within the periphery, central nervous system, and kidneys. Opioids modulate the expression of genes involved in neuroplasticity through epigenetic and possibly RNA modifications, ultimately change the intracellular signaling cascades and dysfunction, and cause long-lasting changes in metabolome. The objective of this study is to identify how opium impacts metabolic pathways to provide markers of abuse, long-term opium addiction, the addiction molecular pathway, and unknown metabolites that are important to differentiation of the study phenotypes. To reach these goals in the present study, the urine specimens of opium abusers and non-users as controls was profiled using an untargeted nuclear magnetic resonance spectroscopy (NMR) metabolomics platform at University of North Carolina at Chapel Hill. The Golestan Cohort Study is conducted in Northeast of Iran to primarily study the risk factors for upper gastrointestinal cancers in this high-risk region, in which about 50,000 volunteers were analyzed for opium users and their mortality. More than 8,000 of participants (17%) age 40-75 reported opium use with a mean duration of 12.7 years. Opium was either smoked or orally consumed. The participants were selected from the cohort stratified by opium use patterns and tobacco use.
Institute
University of North Carolina at Chapel Hill
DepartmentNutrition
LaboratoryUNC-NRI Sumner Lab
Last NameSumner
First NameSusan
Address500 Laureate Way, Kannapolis, NC, 28081, USA
Emailsusan_sumner@unc.edu
Phone919-622-4456
Submit Date2020-12-01
Num Groups2
Total Subjects298
Raw Data AvailableYes
Raw Data File Type(s)pdata, scon2, .par
Analysis Type DetailNMR
Release Date2021-11-24
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8HX30
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001702
Sampleprep Summary:Aliquots of each de-identified sample were shipped to the NIH ERCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 298 study urine samples were thawed at 4°C overnight. Aliquots of 400 µL of study samples were transferred to 2.0mL pre-labeled LoBind Eppendorf tubes. Aliquots of 400 µL of analytical quality control (QC) external pooled urine samples were also transferred to 2.0mL pre-labeled LoBind Eppendorf tubes. All samples were mixed with 300uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH in D2O. The tubes were vortexed for 5 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 10 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4" NMR tubes for data acquisition on a 700 MHz spectrometer.
Processing Storage Conditions:On ice
Extract Storage:Described in summary
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