Summary of Study ST002219

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001417. The data can be accessed directly via it's Project DOI: 10.21228/M8JX39 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Show all samples
Download mwTab file (text) | Download mwTab file(JSON) | Download data files (Contains raw data)

Study ID	ST002219
Study Title	Spatially resolved characterization of tissue metabolic compartments in fasted and high-fat diet livers
Study Summary	Cells adapt their metabolism to physiological stimuli, and metabolic heterogeneity exists between cell types, within tissues, and subcellular compartments. The liver plays an essential role in maintaining whole-body metabolic homeostasis and is structurally defined by metabolic zones. These zones are well-understood on the transcriptomic level, but have not been comprehensively characterized on the metabolomic level. Mass spectrometry imaging (MSI) can be used to map hundreds of metabolites directly from a tissue section, offering an important advance to investigate metabolic heterogeneity in tissues compared to extraction-based metabolomics methods that analyze tissue metabolite profiles in bulk. We established a workflow for the preparation of tissue specimens for matrix-assisted laser desorption/ionization (MALDI) MSI that can be implemented to achieve broad coverage of central carbon, nucleotide, and lipid metabolism pathways. Herein, we used this approach to visualize the effect of nutrient stress and excess on liver metabolism. Our data revealed a highly organized metabolic tissue compartmentalization in livers, which becomes disrupted under high fat diet. Fasting caused changes in the abundance of several metabolites, including increased levels of fatty acids and TCA intermediates while fatty livers had higher levels of purine and pentose phosphate-related metabolites, which generate reducing equivalents to counteract oxidative stress. This spatially conserved approach allowed the visualization of liver metabolic compartmentalization at 30 µm pixel resolution and can be applied more broadly to yield new insights into metabolic heterogeneity in vivo.
Institute	Brigham and Women's Hospital
Department	Brigham and Women's Hospital
Last Name	Stopka
First Name	Sylwia
Address	60 Fenway Rd
Email	sstopka@bwh.harvard.edu
Phone	617-525-9746
Submit Date	2022-05-12
Raw Data Available	Yes
Raw Data File Type(s)	imzML
Analysis Type Detail	MALDI-MS
Release Date	2022-08-18
Release Version	1

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP002311
Sampleprep Summary:	Tissue preparation for MALDI MSI Frozen tissues were placed at -20 °C before sectioning in a Microm HM550 cryostat (Thermo Scientific™). Tissues were sectioned at 10 µm thickness and thaw mounted onto indium-tin-oxide (ITO)-coated slides (Bruker Daltonics) for MALDI MSI analysis with serial sections mounted onto glass slides for histological analyses. The microtome chamber and specimen holder were maintained between -15 °C and -20 °C. Slides were stored at -80 °C until further processing. For desiccation experiments, slides were subjected to desiccation in a tabletop vacuum desiccator before freezing. Matrix deposition A 1,5-Diaminonaphthalene(DAN)-HCl matrix solution was used for all experiments. To generate the hydrochloride derivative of 1,5-DAN, 39.5 mg of 1,5-DAN was dissolved in 500 µL of 1 mol/L hydrochloride solution with 4 mL HPLC-grade water. The solution was sonicated for 20 minutes to dissolve 1,5-DAN, after which 4.5 mL ethanol was added to yield the matrix solution. Matrices were deposited on slides and tissues using a TM-sprayer (HTX imaging, Carrboro, NC). DAN-HCl matrix spray conditions used where: a flow rate of 0.09 mL/min, spray nozzle temperature of 75 °C, and spray nozzle velocity of 1200 mm/min. A four-pass cycle was used with 2 mm track spacing and the nitrogen gas pressure was maintained at 10 psi.

Sampleprep ID:

SP002311

Sampleprep Summary:

Tissue preparation for MALDI MSI Frozen tissues were placed at -20 °C before sectioning in a Microm HM550 cryostat (Thermo Scientific™). Tissues were sectioned at 10 µm thickness and thaw mounted onto indium-tin-oxide (ITO)-coated slides (Bruker Daltonics) for MALDI MSI analysis with serial sections mounted onto glass slides for histological analyses. The microtome chamber and specimen holder were maintained between -15 °C and -20 °C. Slides were stored at -80 °C until further processing. For desiccation experiments, slides were subjected to desiccation in a tabletop vacuum desiccator before freezing. Matrix deposition A 1,5-Diaminonaphthalene(DAN)-HCl matrix solution was used for all experiments. To generate the hydrochloride derivative of 1,5-DAN, 39.5 mg of 1,5-DAN was dissolved in 500 µL of 1 mol/L hydrochloride solution with 4 mL HPLC-grade water. The solution was sonicated for 20 minutes to dissolve 1,5-DAN, after which 4.5 mL ethanol was added to yield the matrix solution. Matrices were deposited on slides and tissues using a TM-sprayer (HTX imaging, Carrboro, NC). DAN-HCl matrix spray conditions used where: a flow rate of 0.09 mL/min, spray nozzle temperature of 75 °C, and spray nozzle velocity of 1200 mm/min. A four-pass cycle was used with 2 mm track spacing and the nitrogen gas pressure was maintained at 10 psi.