Summary of Study ST002396
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001543. The data can be accessed directly via it's Project DOI: 10.21228/M87T4V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002396 |
Study Title | p53 K316P mutation results in increased liver triglyceride levels and increased rates of de novo lipogenesis. |
Study Summary | Our lab generated the p53 K316P mouse, which mimicks a common amino acid change found in bats. The K316P mutation, found in the nuclear localization signal of p53, results in increased cytoplasmic localization of p53. We found that K316P mutant mice develop several metabolic phenotypes, including increased body fat percentage, and increased liver lipid levels. In order to determine the mechanism through which K316P mutation increases liver lipid levels, we performed metabolomic analysis of mouse livers from WT and K316P mutant mice. Mouse livers were isolated from four wild type (WT) and four p53 K316P (M) mice for lipidomic analysis. Samples were isolated and flash frozen in liquid nitrogen. Lipids were then extracted from each liver sample and analyzed using mass spectrometry. |
Institute | University of North Carolina |
Last Name | Sanford |
First Name | Jack |
Address | 450 West Drive, Chapel Hill, NC, 27514 |
jsan4d@email.unc.edu | |
Phone | 3019284726 |
Submit Date | 2022-12-07 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-12-27 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002491 |
Sampleprep Summary: | Mouse liver sections were weighed into Eppendorf tubes. The liver tissue was then mashed with a spatula prior to extraction. The samples were extracted using a liquid liquid partition with water (250 µL), methanol (300 µL), and MTBE (1 mL). Avanti’s deuterated lipid mix, Equisplash, was used as an internal standard. This was spiked into the methanol at 1.5 µg/mL and used for extraction. The extracts were centrifuged at 20,000 rcf for 10 min. The top layer was removed, dried down, and reconstituted in 1 mL of IPA for analysis. |