Summary of Study ST002702

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001673. The data can be accessed directly via it's Project DOI: 10.21228/M8FQ4P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002702
Study Title	A targeted metabolomics approach for sepsis-induced ARDS and its subphenotypes
Study Summary	Acute respiratory distress syndrome (ARDS) is etiologically and clinically a heterogeneous disease. Its diagnostic characteristics and subtype classification, and the application of these features to treatment, have been of considerable interest. Metabolomics is becoming important for identifying ARDS biology and distinguishing its subtypes. This study aimed to identify metabolites that could distinguish sepsis-induced ARDS patients from non-ARDS controls, using a targeted metabolomics approach, and to identify whether sepsis-induced direct and sepsis-induced indirect ARDS are metabolically distinct groups, and if so, confirm their metabolites and associated pathways.
Institute	Asan Medical Center
Last Name	Yoo
First Name	Hyun Ju
Address	88, Olympic-ro 43-gil, Songpa-gu
Email	d131108@ulsan.ac.kr
Phone	0230104029
Submit Date	2023-05-15
Raw Data Available	Yes
Raw Data File Type(s)	OTHER
Analysis Type Detail	GC-MS/LC-MS
Release Date	2023-07-06
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002813
Sampleprep Summary:	Sample solutions were prepared by using commonly used liquid-liquid extaction procedure known as Bligh/Dyer mthod with minor modifications.(Can.J.Biochem.Physiol. 37:911-917) Briefly, 400 μL of chloroform/methanol (1/2, v/v) was added to each sample solution and mixed well. The solution was centrifuged for 15 min at 14000 rpm. After centrifugation, a thick precipitate containing macromolecules was found between the aqueous upper layer and the organic lower layer. Polar metabolites were contained in the upper aqueous phase. The collected volume from each layer were generally the same, however sometimes any specific sample had thicker interface between organic and aqueous layer, which resulted in little variation of the recovered volumes. However, internal standards added prior to sample preparation should correct this variation. The aqueous phases were dried under vacuum and stored at -20℃ until further analysis. The dried matter from the aqueous solutions was reconstituted with 50 μL of H2O/MeOH (50/50 v/v) prior to liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis. For amino acids, 10 μL out of the total 50 μL reconstituted aqueous phase was used for chemical derivatization of amino acids using phenylisothiocyanate.For S1P and free fatty acids, separate serum samples were used. Details can be found in attached method file.

Sampleprep ID:

SP002813

Sampleprep Summary:

Sample solutions were prepared by using commonly used liquid-liquid extaction procedure known as Bligh/Dyer mthod with minor modifications.(Can.J.Biochem.Physiol. 37:911-917) Briefly, 400 μL of chloroform/methanol (1/2, v/v) was added to each sample solution and mixed well. The solution was centrifuged for 15 min at 14000 rpm. After centrifugation, a thick precipitate containing macromolecules was found between the aqueous upper layer and the organic lower layer. Polar metabolites were contained in the upper aqueous phase. The collected volume from each layer were generally the same, however sometimes any specific sample had thicker interface between organic and aqueous layer, which resulted in little variation of the recovered volumes. However, internal standards added prior to sample preparation should correct this variation. The aqueous phases were dried under vacuum and stored at -20℃ until further analysis. The dried matter from the aqueous solutions was reconstituted with 50 μL of H2O/MeOH (50/50 v/v) prior to liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis. For amino acids, 10 μL out of the total 50 μL reconstituted aqueous phase was used for chemical derivatization of amino acids using phenylisothiocyanate.For S1P and free fatty acids, separate serum samples were used. Details can be found in attached method file.