Summary of Study ST002804

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001751. The data can be accessed directly via it's Project DOI: 10.21228/M8CH9W This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002804
Study TitleMetabolic Adaptations of Microbacterium sediminis YLB-01 for Survival in the Challenging High-Pressure of the Deep Sea
Study SummaryBy using NMR-based metabolomic analysis, we investigated the metabolic adaptations of Microbacterium sediminis YLB-01 in response to high-pressure conditions. We recorded the 600 MHz 1D 1H-NMR spectra on aqueous extracts from YLB-01 cells, and then assigned resonances of 31 metabolites. The distinct metabolic separation between the HPLT and NPLT groups highlighted the significant effect of high-pressure treatment on the metabolism of YLB-01 cells.
Xiamen University
Last NameQiu
First NameXu
AddressNo. 422, Siming South Road, Xiamen, Fujian, China.
Submit Date2023-06-18
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2023-08-22
Release Version1
Xu Qiu Xu Qiu application/zip

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Sample Preparation:

Sampleprep ID:SP002917
Sampleprep Summary:After culturing the YLB-01 cells, each 100 mL of the culture was transferred into a 250-mL centrifuge bottle and centrifuged at 4°C (6000 g, 5 min). The supernatant was carefully decanted, and the cell pellets were rapidly cooled to -40°C using 100 mL of a buffer composed of a 3:2 methanol/water mixture containing 0.85% (wt/vol) NaCl. The mixture was centrifuged again at 4°C (6000 g, 5 min). The cell pellets were washed three times with 5 mL of cold phosphate-buffered saline (PBS) and centrifuged at 4°C (6000 g, 5 min) after each wash. Finally, the cell pellets were stored at -80°C until further use. Initially, 600 μL of a cold extraction buffer consisting of a 1:1 mixture of distilled water and acetonitrile was added to homogenize the samples. The mixtures were then sonicated on wet ice for 180 cycles, with each cycle consisting of 2 seconds of ultrasound followed by a 3-second pause. After centrifugation at 4°C (12000 g, 10 min), the supernatants were collected and lyophilized, resulting in an extract powder that was stored at -80°C for further analysis.
Processing Storage Conditions:Described in summary
Extract Storage:Described in summary