Summary of Study ST002831
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001773. The data can be accessed directly via it's Project DOI: 10.21228/M8J420 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002831 |
Study Title | Folate depletion upregulates heme synthesis |
Study Summary | Culture of K562 cells in RPMI media containing 2,000 nM folic acid or 100 nM folic acid for 6 days followed my polar metabolomics. |
Institute | Boston Children's Hospital, Harvard Medical School |
Department | pathology |
Laboratory | Kanarek Lab |
Last Name | Kanarek |
First Name | Naama |
Address | Enders 1116.2, 300 Longwood Ave, Boston, MA 02115 |
naama.kanarek@childrens.harvard.edu | |
Phone | 6173557433 |
Submit Date | 2023-08-22 |
Num Groups | 2 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-02-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP002946 |
Sampleprep Summary: | Pellet was resuspended in extraction buffer (80% Methanol, 25 mM Ammonium Acetate and 2.5 mM Na-Ascorbate prepared in LC-MS water, supplemented with isotopically-labelled amino acid standards [Cambridge Isotope Laboratories, MSK-A2-1.2], aminopterin, and reduced glutathione standard [Cambridge Isotope Laboratories, CNLM-6245-10]). Samples were vortexed for 10 sec, then centrifuged for 10 minutes at 18,000 g to pellet cell debris. The supernatant was divided into two tubes and dried on ice using a liquid nitrogen dryer. One tube of dried sample was used for polar metabolite detection. Dried samples were resuspended in 25 μL water |