Summary of Study ST003003

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001872. The data can be accessed directly via it's Project DOI: 10.21228/M8R41G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003003
Study Title	Gut microbiota and metabolites in estrus cycle and their changes in a menopausal transition rat model with typical neuroendocrine aging
Study Summary	Neuroendocrine alterations in the mid-life hypothalamus coupled with reproductive decline herald the initiation of menopausal transition. The certain feature and contribution of gut microflora and metabolites to neuroendocrine changes in the menopausal transition remain largely unknown. Fecal samples of rats experiencing different reproductive stages were collected and processed for 16S rRNA and liquid chromatography-mass spectrometry sequencing. The differences of gut microbiota and metabolites between young and middle-aged rats during proestrus and diestrus were analyzed and their relationships to neuroendocrine aging were then examined. This study documents specific gut microbial composition changes and concomitant shifting trends of metabolites during menopausal transition, which may initiate the gut-brain dysfunction in neuroendocrine aging.
Institute	Fudan University
Last Name	Dai
First Name	Ruoxi
Address	128 Shenyang Road
Email	rqdai21@m.fudan.edu.cn
Phone	+86-21-33189900
Submit Date	2023-12-13
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2023-12-18
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003123
Sampleprep Summary:	The fecal samples weighed 60 mg were transferred to 1.5-mL EP tubes with addition of 20 μL L-2-chlorophenylalanine (0.3 mg/mL) and 20 μL Lyso PC17:0 (0.01 mg/ml) in methanol and 600 μL methanol/water, 4:1 (v/v). After pre-cooled at -20℃ for 2 min, the samples were ground at 60 Hz for 2 min by steel beads, sonicated for 10 min and centrifuged for 10 min at 13,000 rpm, 4℃ following standing still at -20℃ for 30 min. 300 μL of supernatants were dried and then resolved in 400 μL methanol/water, 1:4 (v/v). The mixtures were vortexed for 30 s, sonicated for 2 min and centrifuged at 13,000 rpm, 4℃ for 10 min. 150 μL supernatant was aspirated with syringes and filtered through 0.22 μm organic phase pinhole filters. The extracts were transferred to LC injection vials and store at -80℃ until LC-MS analysis.

Sampleprep ID:

SP003123

Sampleprep Summary:

The fecal samples weighed 60 mg were transferred to 1.5-mL EP tubes with addition of 20 μL L-2-chlorophenylalanine (0.3 mg/mL) and 20 μL Lyso PC17:0 (0.01 mg/ml) in methanol and 600 μL methanol/water, 4:1 (v/v). After pre-cooled at -20℃ for 2 min, the samples were ground at 60 Hz for 2 min by steel beads, sonicated for 10 min and centrifuged for 10 min at 13,000 rpm, 4℃ following standing still at -20℃ for 30 min. 300 μL of supernatants were dried and then resolved in 400 μL methanol/water, 1:4 (v/v). The mixtures were vortexed for 30 s, sonicated for 2 min and centrifuged at 13,000 rpm, 4℃ for 10 min. 150 μL supernatant was aspirated with syringes and filtered through 0.22 μm organic phase pinhole filters. The extracts were transferred to LC injection vials and store at -80℃ until LC-MS analysis.