Summary of Study ST003151
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001941. The data can be accessed directly via it's Project DOI: 10.21228/M8TM76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003151 |
| Study Title | BT474 breast cancer cell line grown in 20% D2O-containing RPMI-1640 medium treated with Fasnall and GSK2194069 |
| Study Type | Free fatty acid analysis, D2O tracing |
| Study Summary | BT474 breast cancer cell line grown in 20% D2O-containing medium treated with Fasnall and GSK2194069. Cells were grown for 24 h in RPMI-1640 with 10% dialyzed FBS. |
| Institute | Wistar Institute |
| Department | Molecular and Cellular Oncogenesis Program, Ellen and Ronald Caplan Cancer Center |
| Laboratory | Schug's Lab |
| Last Name | Mukha |
| First Name | Dzmitry |
| Address | 3601 Spruce St, Philadelphia, PA 19104, USA |
| dmukha@wistar.org | |
| Phone | 2154956903 |
| Submit Date | 2024-03-09 |
| Num Groups | 7 |
| Total Subjects | 21 |
| Publications | Submission Pending |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML, raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-04-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP003275 |
| Sampleprep Summary: | Sample preparation for LC-MS free fatty acid analysis Cells were seeded in 10 cm Petri dishes. At collection, cells were washed with PBS three times. Then, 1 ml of methanol was added and cells were scraped from the surface. All content of the plate was transferred into 13 x 100 mm Pyrex glass tubes. Lipids were extracted by the Folch method. Dried lipids were redissolved in 1 ml of 0.3 M KOH solution in 90% methanol and incubated at 85 °C for 1 h. Then, 100 µl of formic acid were added, followed by 800 µl of hexane for extraction. The hexane phase was transferred to glass LC-MS vials and dried under the stream of nitrogen. Samples were redissolved in 1 ml of 1:1 methanol:isopropanol. |
| Sampleprep Protocol Filename: | DM_free_fatty_acid_analysis_samples.txt |
| Processing Method: | Lipid saponification in 0.3 M KOH |
| Extraction Method: | 100% methanol |
| Extract Enrichment: | None |
| Extract Cleanup: | None |
| Extract Storage: | -80℃ |
| Sample Resuspension: | None |
| Sample Derivatization: | None |
| Sample Spiking: | None |
| Subcellular Location: | Cellular lipids |
