Summary of Study ST000540
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000395. The data can be accessed directly via it's Project DOI: 10.21228/M80G60 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000540 |
| Study Title | Kidney tissue metabolomic profiling of diabetic nephropathy in the steptozotocin induced type-1 diabetes mouse model. |
| Study Type | Metabolomics |
| Study Summary | This metabolomics study evaluated kidney tissue from wild-type and meprin β knockout mice after induction of diabetes with streptozotocin or treatment with sodium citrate control to understand how these factors influence the metabotype. |
| Institute | RTI International |
| Department | Discovery-Science-Technology |
| Laboratory | NIH Eastern Regional Comphrehensive Metabolomics Resource Core at UNC Chapel Hill (ERCMRC) |
| Last Name | Sumner |
| First Name | Susan |
| Address | 3040 E Cornwallis Road, Research Triangle Park, NC 27709 |
| susan_sumner@unc.edu | |
| Phone | 704-250-5000 |
| Submit Date | 2017-01-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR000576 |
| Treatment Summary: | Type 1 diabetes was induced at age 8 weeks. The mice were fasted for 6 hours prior to being injected with low dose STZ (50 mg/kg body weight) in sodium citrate buffer (10 mmol/L, pH 4.5) daily for 5 days following the protocols described by Tesch and Allen and recommended by the Animal Models of Diabetic Complications Consortium (AMDCC; amdcc.org)1. Control mice were injected with equivalent volumes of the sodium citrate buffer. Diabetes was confirmed by measuring fasting blood glucose levels using a standard glucose meter at 10 days post-STZ injection. Mice with a fasting glucose level ≥280 mg/dL were considered diabetic. STZ-injected mice with a fasting blood glucose of <280 mg/dL (15mmol/L) were culled and eliminated from the study. |
| Treatment Compound: | streptozotocin |
| Treatment Dose: | 50 mg/kg body weight |
| Treatment Doseduration: | daily; 5 days |
| Treatment Vehicle: | sodium citrate |
| Animal Endp Tissue Coll List: | plasma, urine, kidney tissue |
