Summary of Study ST001210

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000813. The data can be accessed directly via it's Project DOI: 10.21228/M8KX27 This work is supported by NIH grant, U2C- DK119886.


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Study IDST001210
Study TitleComprehensive UHPLC-MS/MS lipidomics profiling to study effects of betulin on keratinocytes
Study SummaryLipidomics analysis of betulin in human primary keratinocytes to monitor alterations in the lipid profiles induced by treatment with betulin
Eberhard Karls University of Tübingen
DepartmentInstitute of Pharmaceutical Sciences
LaboratoryPharmaceutical (Bio-)Analysis
Last NameCalderon
First NameCarlos
AddressAuf der Morgenstelle 8 (Haus B), D-72076, Tuebingen, baden württemberg, 72076, Germany
Phone+49 (0)7071 29 74009
Submit Date2019-07-05
Num Groups2
Total Subjects20
Study CommentsEberhard-Karls-University Tuebingen
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Carlos Calderon Carlos Calderon application/zip

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Treatment ID:TR001292
Treatment Summary:Cells were split when they reached a confluency of 80 %. Passage 3 or 4 were used. Cells were plated in 20 Petri dishes (10 cm2, 1 x 106 cells/ per dish) and cultivated for 4 days in 10 mL of the above mentioned medium for adherence. 10 dishes were incubated with 1.95 µM betulin (10 µL of the 1.95 mM stock solution in DMSO) for 8 hrs prior to removal of the medium and the remaining ones were used as control. Control samples were treated with 10 µL DMSO. The concentration of 1.95 µM of betulin was used, as this concentration has shown effects in our previous studies on the molecular wound healing effect [1]. On day 4, cells were incubated with 3 ml of trypsin (0.05 %) at 37°C. After 5 min cells were washed by adding 7 mL medium. The suspension was transferred into 15 mL falcon tubes and centrifuged for 5 min at 4°C and 1.200 rpm, respectively. The supernatant was withdrawn and the remaining cell pellets were washed and then frozen at -20°C in the falcon tubes until extraction.
Treatment Protocol Filename:ccalcas_Extraction_and_treatment.pdf