Summary of Study ST001979

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001255. The data can be accessed directly via it's Project DOI: 10.21228/M8GT41 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001979
Study TitleAnti-oxidation metabolism measurement in mammalian cells and tissues by quantitative LC/MS method (VI)
Study SummaryAnti-oxidation metabolism measurement in mouse CSF by quantitative LC/MS method.
Institute
Boston Children's Hospital, Harvard Medical School
Last NamePetrova
First NameBoryana
Address300 Longwood Ave
Emailboryana.petrova@childrens.harvard.edu
Phone6173557433
Submit Date2021-09-15
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-08-29
Release Version1
Boryana Petrova Boryana Petrova
https://dx.doi.org/10.21228/M8GT41
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Treatment:

Treatment ID:TR002071
Treatment Summary:Mice were treated with PBS, PBS+ MTX, Null GFP with and without MTX, CAG-GFP with and without MTX, and CMV-GFP with and without MTX. buffers from study design were used for all respective samples. CD-1 mice (6-8 weeks) or Sprague Dawley rats (8 weeks) were given a single intravenous injection of 75 mg/kg MTX (Fisher Scientific, AAJ66364MD) dissolved in 0.9 % NaCl (Mountainside Medical, 0409-4888-10). MTX was freshly prepared for each experiment. MTX in mouse serum or CSF was assessed by MTX ELISA according to the manufacturer's instructions (Enzo Life Sciences, 142-0001).
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