Summary of Study ST002954
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001837. The data can be accessed directly via it's Project DOI: 10.21228/M88B0T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002954 |
| Study Title | Metabolite flux from temperature-acclimated diatom strains (drawdown experiment) |
| Study Summary | The temperature increase occurring in the surface ocean has fundamental implications for physiological rates and processes of marine microbes. Here we asked whether the temperature at which a marine diatom strain is acclimated affects carbon transfer to a co-cultured heterotrophic bacterium. Model systems were established in which the diatom Thalassiosira pseudonana was acclimated for three months at temperatures below (14°C), equal to (20°C), and above (28°C) the temperature of optimal growth, and then inoculated with the heterotrophic bacterium Ruegeria pomeroyi. This deposition is for results obtained from a drawdown experiment of phytoplankton metabolites using R. pomeroyi conducted during this study. |
| Institute | University of Georgia |
| Laboratory | Moran Lab, Edison Lab |
| Last Name | Uchimiya |
| First Name | Mario |
| Address | 315 Riverbend Rd, Athens, GA, 30602, USA |
| mario.uchimiya@uga.edu | |
| Phone | (706) 542-8387 |
| Submit Date | 2023-10-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2023-11-22 |
| Release Version | 1 |
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Treatment:
| Treatment ID: | TR003076 |
| Treatment Summary: | Axenic cultures (1 L in L1 media, 35 ppt) of the diatom Thalassiosira pseudonana CCMP1335 were grown at different temperatures of 14, 20, and 28°C under 120 µmol photons m-2 s-1 (ULM-500 Light Meter, Walz) and a 16:8 h light:dark cycle (four replicates for each treatment). They were harvested in late exponential growth phase by filtration onto 2.0 µm Isopore filters (1 L) and stored at -20°C for later experimentation. Diatom cells were rinsed from filters into 10 mL sterile L1 media, sonicated to lyse cells for 20 min, and passed through a pre-combusted GF/F filter. R. pomeroyi was inoculated into 2.7 mL of the endometabolome samples (3 x 107 cells mL-1) and grown for 10 h. |
| Treatment Protocol Filename: | 3_Treatment protocol_UGA_temp_Oct2023_drawdown.docx |
