Summary of Study ST003172

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001972. The data can be accessed directly via it's Project DOI: 10.21228/M8TJ0V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003172
Study Title	Untargeted Metabolomic Profile Of Chili Pepper (Capsicum Chinensed) Developmental Cycle
Study Summary	To explore the temporal dynamics of metabolites in chili peppers at various developmental stages, we employed a non-targeted metabolomics method based on liquid chromatography-mass spectrometry (LC-MS). The metabolome data collection began on the day of flowering (0 days post-anthesis), and continued for 7, 16, 30, 50, 55, and 60 DPA. This comprehensive dataset paved the way for future studies on metabolite changes and biological processes in chili peppers throughout their life cycle.
Institute	University of Alberta
Department	Department of Chemistry
Last Name	Yang
First Name	Bowen
Address	11227 Saskatchewan Drive , Edmonton, Alberta, Canada T6G 2G2
Email	by8@ualberta.ca
Phone	8259758666
Submit Date	2024-04-13
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2024-04-18
Release Version	1

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Treatment:

Treatment ID:	TR003300
Treatment Summary:	Metabolic profiling data from chili peppers (Capsicum chinese L.) was collected. Pepper seedlings were grown in a greenhouse of Peking University Institute of Advanced Agricultural Sciences with a controlled environment of 25°C temperature, a light-dark cycle of 16 hours light and 8 hours dark, and 70% relative humidity. The fruits of peppers were sampled at seven distinct time points, starting from the day of flowering, i.e. 0 day post-anthesis (DPA) during which flowers were collected. Following this, fruits were harvested on days 7, 16, 30, 50, 55, and 60 DPA. The samples were ground and freeze-dried in liquid nitrogen, followed by extraction using 1.0 mL of 70% aqueous methanol for every 50 mg of the sample, and an ultrasonic process was employed for 30 minutes. The preparation of standards was conducted as follows: a mixed standard in the range of 20-50μg/mL was prepared using methanol of MS grade. For the amino acid standard solution, a 1mg/mL stock solution was prepared in water and subsequently diluted with 50% methanol to achieve a concentration of 50μg/mL. Three biological replicates were used metabolome analysis.

Treatment ID:

TR003300

Treatment Summary:

Metabolic profiling data from chili peppers (Capsicum chinese L.) was collected. Pepper seedlings were grown in a greenhouse of Peking University Institute of Advanced Agricultural Sciences with a controlled environment of 25°C temperature, a light-dark cycle of 16 hours light and 8 hours dark, and 70% relative humidity. The fruits of peppers were sampled at seven distinct time points, starting from the day of flowering, i.e. 0 day post-anthesis (DPA) during which flowers were collected. Following this, fruits were harvested on days 7, 16, 30, 50, 55, and 60 DPA. The samples were ground and freeze-dried in liquid nitrogen, followed by extraction using 1.0 mL of 70% aqueous methanol for every 50 mg of the sample, and an ultrasonic process was employed for 30 minutes. The preparation of standards was conducted as follows: a mixed standard in the range of 20-50μg/mL was prepared using methanol of MS grade. For the amino acid standard solution, a 1mg/mL stock solution was prepared in water and subsequently diluted with 50% methanol to achieve a concentration of 50μg/mL. Three biological replicates were used metabolome analysis.