Summary of Study ST002118

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001343. The data can be accessed directly via it's Project DOI: 10.21228/M8439K This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002118
Study TitleExposures to Metals in Pregnant Women and the Impact on Fetal Development and Birth Outcomes in Suriname
Study TypeC18 Reversed-Phase Broad Spectrum Metabolomics
Study SummaryThe Caribbean Consortium for Environmental and Occupational Health, a NIH-funded integrated research and research training program, started in 2015. The research component is a population-based prospective longitudinal environmental epidemiologic cohort study addressing the potential adverse impact of chemical and non-chemical environmental exposures in mother/child dyads in Suriname. The study determines associations between exposures to neurotoxicants (metals and pesticides) and essential elements and non-chemical stressors in pregnant women and the impact on birth and neurodevelopmental outcomes. The study population consists of culturally diverse pregnant women (n=1143; ages: 16-49 years) and their babies/children (n=1069). Data collection takes place twice prenatally, at birth, 12, 36, and 48 months. Through HHEAR, targeted and untargeted (metabolomics) analyses will characterize exposure to metals in a sub-cohort of pregnant women for whom exposure data are not yet available. This expanded exposure analysis will enable a more comprehensive cumulative risk assessment of adverse birth and neurodevelopmental outcomes in the overall cohort.
Institute
University of Pittsburgh
DepartmentDepartment of Environmental and Occupational Health
Last NameLichtveld
First NameMaureen
Address130 DeSoto Street, University of Pittsburgh, Pittsburgh, PA 15261
Emailmlichtve@pitt.edu
Phone412-624-6818
Submit Date2022-03-22
Total Subjects668
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2023-03-22
Release Version1
Maureen Lichtveld Maureen Lichtveld
https://dx.doi.org/10.21228/M8439K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001343
Project DOI:doi: 10.21228/M8439K
Project Title:Exposures to Metals in Pregnant Women and the Impact on Fetal Development and Birth Outcomes in Suriname
Project Type:C18 Reversed-Phase Broad Spectrum Metabolomics
Project Summary:The Caribbean Consortium for Environmental and Occupational Health, a NIH-funded integrated research and research training program, started in 2015. The research component is a population-based prospective longitudinal environmental epidemiologic cohort study addressing the potential adverse impact of chemical and non-chemical environmental exposures in mother/child dyads in Suriname. The study determines associations between exposures to neurotoxicants (metals and pesticides) and essential elements and non-chemical stressors in pregnant women and the impact on birth and neurodevelopmental outcomes. The study population consists of culturally diverse pregnant women (n=1143; ages: 16-49 years) and their babies/children (n=1069). Data collection takes place twice prenatally, at birth, 12, 36, and 48 months. Through HHEAR, targeted and untargeted (metabolomics) analyses will characterize exposure to metals in a sub-cohort of pregnant women for whom exposure data are not yet available. This expanded exposure analysis will enable a more comprehensive cumulative risk assessment of adverse birth and neurodevelopmental outcomes in the overall cohort.
Institute:NC HHEAR Hub
Department:Untargeted Analysis
Laboratory:Sumner Lab
Last Name:Li
First Name:Yuan
Address:Nutrition Research Institute, UNC-CH,500 Laureate Way, Kannapolis, NC 28081
Email:yuanyli4@unc.edu
Phone:984-377-0693
Funding Source:The Caribbean Consortium for Environmental and Occupational Health is funded by the Fogarty International Center of the National Institutes of Health (U01TW010087, U2RTW010104). Untargeted metabolomics analyses were conducted at the North Carolina Human Health Exposure Analysis Resource Hub: Research Triangle Institute: Fennell T, University of North Carolina at Chapel Hill: Sumner S, University of North Carolina at Charlotte: Du X (U2C ES030857), and support provided by the Human Health Exposure Analysis Resource Coordinating Center: Westat, Inc.: O’Brien B (U24 ES026539), and the Human Health Exposure Analysis Data Center: Icahn School of Medicine at Mount Sinai: Teitelbaum S (U2CES026555)

Subject:

Subject ID:SU002203
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA203144Lich_HHEAR_30HHEAR Reference Material
SA203145Lich_HHEAR_31HHEAR Reference Material
SA203146Lich_HHEAR_34HHEAR Reference Material
SA203147Lich_HHEAR_29HHEAR Reference Material
SA203148Lich_HHEAR_33HHEAR Reference Material
SA203149Lich_HHEAR_27HHEAR Reference Material
SA203150Lich_HHEAR_24HHEAR Reference Material
SA203151Lich_HHEAR_25HHEAR Reference Material
SA203152Lich_HHEAR_26HHEAR Reference Material
SA203153Lich_HHEAR_35HHEAR Reference Material
SA203154Lich_HHEAR_28HHEAR Reference Material
SA203155Lich_HHEAR_37HHEAR Reference Material
SA203156Lich_HHEAR_43HHEAR Reference Material
SA203157Lich_HHEAR_44HHEAR Reference Material
SA203158Lich_HHEAR_45HHEAR Reference Material
SA203159Lich_HHEAR_46HHEAR Reference Material
SA203160Lich_HHEAR_42HHEAR Reference Material
SA203161Lich_HHEAR_41HHEAR Reference Material
SA203162Lich_HHEAR_23HHEAR Reference Material
SA203163Lich_HHEAR_38HHEAR Reference Material
SA203164Lich_HHEAR_39HHEAR Reference Material
SA203165Lich_HHEAR_40HHEAR Reference Material
SA203166Lich_HHEAR_36HHEAR Reference Material
SA203167Lich_HHEAR_22HHEAR Reference Material
SA203168Lich_HHEAR_7HHEAR Reference Material
SA203169Lich_HHEAR_8HHEAR Reference Material
SA203170Lich_HHEAR_9HHEAR Reference Material
SA203171Lich_HHEAR_10HHEAR Reference Material
SA203172Lich_HHEAR_6HHEAR Reference Material
SA203173Lich_HHEAR_5HHEAR Reference Material
SA203174Lich_HHEAR_1HHEAR Reference Material
SA203175Lich_HHEAR_2HHEAR Reference Material
SA203176Lich_HHEAR_3HHEAR Reference Material
SA203177Lich_HHEAR_4HHEAR Reference Material
SA203178Lich_HHEAR_11HHEAR Reference Material
SA203179Lich_HHEAR_12HHEAR Reference Material
SA203180Lich_HHEAR_18HHEAR Reference Material
SA203181Lich_HHEAR_19HHEAR Reference Material
SA203182Lich_HHEAR_20HHEAR Reference Material
SA203183Lich_HHEAR_21HHEAR Reference Material
SA203184Lich_HHEAR_17HHEAR Reference Material
SA203185Lich_HHEAR_16HHEAR Reference Material
SA203186Lich_HHEAR_13HHEAR Reference Material
SA203187Lich_HHEAR_14HHEAR Reference Material
SA203188Lich_HHEAR_15HHEAR Reference Material
SA203189Lich_HHEAR_47HHEAR Reference Material
SA203190Lich_HHEAR_32HHEAR Reference Material
SA203191Lich_HHEAR_64HHEAR Reference Material
SA203192Lich_HHEAR_62HHEAR Reference Material
SA203193Lich_HHEAR_61HHEAR Reference Material
SA203194Lich_HHEAR_60HHEAR Reference Material
SA203195Lich_HHEAR_65HHEAR Reference Material
SA203196Lich_HHEAR_66HHEAR Reference Material
SA203197Lich_HHEAR_48HHEAR Reference Material
SA203198Lich_HHEAR_68HHEAR Reference Material
SA203199Lich_HHEAR_67HHEAR Reference Material
SA203200Lich_HHEAR_59HHEAR Reference Material
SA203201Lich_HHEAR_63HHEAR Reference Material
SA203202Lich_HHEAR_52HHEAR Reference Material
SA203203Lich_HHEAR_53HHEAR Reference Material
SA203204Lich_HHEAR_51HHEAR Reference Material
SA203205Lich_HHEAR_58HHEAR Reference Material
SA203206Lich_HHEAR_49HHEAR Reference Material
SA203207Lich_HHEAR_54HHEAR Reference Material
SA203208Lich_HHEAR_50HHEAR Reference Material
SA203209Lich_HHEAR_57HHEAR Reference Material
SA203210Lich_HHEAR_56HHEAR Reference Material
SA203211Lich_HHEAR_55HHEAR Reference Material
SA203212Lich_NIST_16NIST Reference Material
SA203213Lich_NIST_15NIST Reference Material
SA203214Lich_NIST_14NIST Reference Material
SA203215Lich_NIST_18NIST Reference Material
SA203216Lich_NIST_13NIST Reference Material
SA203217Lich_NIST_20NIST Reference Material
SA203218Lich_NIST_23NIST Reference Material
SA203219Lich_NIST_24NIST Reference Material
SA203220Lich_NIST_22NIST Reference Material
SA203221Lich_NIST_21NIST Reference Material
SA203222Lich_NIST_12NIST Reference Material
SA203223Lich_NIST_19NIST Reference Material
SA203224Lich_NIST_17NIST Reference Material
SA203225Lich_NIST_4NIST Reference Material
SA203226Lich_NIST_2NIST Reference Material
SA203227Lich_NIST_1NIST Reference Material
SA203228Lich_NIST_11NIST Reference Material
SA203229Lich_NIST_5NIST Reference Material
SA203230Lich_NIST_3NIST Reference Material
SA203231Lich_NIST_6NIST Reference Material
SA203232Lich_NIST_10NIST Reference Material
SA203233Lich_NIST_9NIST Reference Material
SA203234Lich_NIST_8NIST Reference Material
SA203235Lich_NIST_7NIST Reference Material
SA203236Lich_SP_23Study Pool
SA203237Lich_SP_24Study Pool
SA203238Lich_SP_22Study Pool
SA203239Lich_SP_19Study Pool
SA203240Lich_SP_25Study Pool
SA203241Lich_SP_20Study Pool
SA203242Lich_SP_21Study Pool
SA203243Lich_SP_31Study Pool
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Collection:

Collection ID:CO002196
Collection Summary:The Caribbean Consortium for Environmental and Occupational Health, a NIH-funded integrated research and research training program, started in 2015. The research component is a population-based prospective longitudinal environmental epidemiologic cohort study addressing the potential adverse impact of chemical and non-chemical environmental exposures in mother/child dyads in Suriname. The study determines associations between exposures to neurotoxicants (metals and pesticides) and essential elements and non-chemical stressors in pregnant women and the impact on birth and neurodevelopmental outcomes. The study population consists of culturally diverse pregnant women (n=1143; ages: 16-49 years) and their babies/children (n=1069). Data collection takes place twice prenatally, at birth, 12, 36, and 48 months. Through HHEAR, targeted and untargeted (metabolomics) analyses will characterize exposure to metals in a sub-cohort of pregnant women for whom exposure data are not yet available. This expanded exposure analysis will enable a more comprehensive cumulative risk assessment of adverse birth and neurodevelopmental outcomes in the overall cohort.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002215
Treatment Summary:N/A

Sample Preparation:

Sampleprep ID:SP002209
Sampleprep Summary:Study serum samples (50 µL) and total study pool samples (50 µL) were prepared and shipped from Tulane Univeristy to the NC HHEAR Hub on dry ice. Aliquots of HHEAR reference plasma (50 µL each) and NIST serum (909C) reference material (50 µL each) were provided by the NC HHEAR Hub. All samples were stored at -80° C until lab analysis. All samples were thawed at 4 °C overnight a day before sample preparation. The same volume (50 µL) of LC-MS grade water was used as blank. Samples, including study samples, total study pool samples, HHEAR reference material, NIST reference material, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm in room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, and then dried by a SpeedVac overnight. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts, and the samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature. All samples were centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS.
Processing Storage Conditions:On ice
Extraction Method:Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard
Extract Storage:-80℃
Sample Resuspension:Water-Methanol (95:5, v/v)
Sample Spiking:Tryptophan-d5 stock solution at 500 ng/mL

Combined analysis:

Analysis ID AN003466
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE
Units Area

Chromatography:

Chromatography ID:CH002560
Chromatography Summary:Reverse phase
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um)
Column Pressure:6000-10000 psi
Column Temperature:50
Flow Gradient:Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5
Flow Rate:0.4 ml/min
Injection Temperature:8
Internal Standard:Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Randomization Order:Randomized
Chromatography Type:Reversed phase

MS:

MS ID:MS003227
Analysis ID:AN003466
Instrument Name:Thermo Q Exactive HF-X Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Instrument: Thermo Q Exactive HFx Software: Xcalibur 4.1.31.9 for data acquisition; Progenesis QI 2.1 for data preprocessing
Ion Mode:POSITIVE
Capillary Temperature:320 °C
Collision Energy:20-45, ramp
Collision Gas:N2
Dry Gas Flow:55
Dry Gas Temp:400°C
Fragmentation Method:CID
Mass Accuracy:5 ppm
Spray Voltage:3.5 KV
Dataformat:Profile
Desolvation Gas Flow:55
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