Summary of Study ST000069

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000066. The data can be accessed directly via it's Project DOI: 10.21228/M8QG6H This work is supported by NIH grant, U2C- DK119886.

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Study IDST000069
Study TitleMetabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice (via blood and tissue)
Study SummaryLung samples were obtained at 15 weeks from 56 mice that had received either chow, a low fat diet, or a high fat diet and that were uninfected, 4 days post-infection, or 8 days post-infection.
Institute
University of North Carolina
DepartmentSystems and Translational Sciences
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2014-06-14
Num Groups9
Total Subjects55
Raw Data AvailableNo
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2015-06-01
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8QG6H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000066
Project DOI:doi: 10.21228/M8QG6H
Project Title:Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice
Project Type:Effects and role of obesity on the severity of influenza
Project Summary:During the 2009 H1N1 influenza pandemic outbreak, obese individuals were reported to be at greater risk for morbidity and mortality from pandemic infection. However, the mechanisms contributing to greater influenza severity in infected obese individuals remain unclear. Given that one in ten individuals is obese, and worldwide influenza outbreaks are a consistent public health burden, garnering a better understanding of the pathways and mechanisms contributing to greater influenza severity in the obese is essential for limiting influenza infection mortality in this at-risk population. Closely paralleling pH1N1 infection outcome in humans, obese mice exhibit increased morbidity and mortality following pH1N1 infection. In mice, obesity impairs the function of natural killer cells, dendritic cells, macrophage, B cells and memory T cells. Further, several analyses of lung antiviral responses revealed that obese mice have greater lung damage, lung immune cell infiltration and impaired lung healing after infection. Nevertheless, it remains unclear how altered immune cell function contributes to greater lung damage and increased infection severity in obese mice. Metabolomics will be used to dissect the metabolic consequences of obesity on the immune response to pH1N1 infection. We will compare metabolic profiles of lung-specific and peripheral samples from uninfected and infected lean and obese mice during early and late phases of influenza immunity.
Institute:University of North Carolina at Chapel Hill
Department:Department of Nutrition
Last Name:Beck
First Name:Melinda
Address:2303 MHRB, CB #7461, UNC, Chapel Hill NC 27599
Email:melinda_beck@unc.edu
Phone:919-966-6809

Subject:

Subject ID:SU000088
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:approx. 15-16 weeks
Gender:male
Animal Animal Supplier:The Jackson Laboratory
Animal Feed:high fat, low fat, or chow
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA003603LPDAY4001_POS_RF4
SA003604LPDAY4002_POS_RF4
SA003605LPDAY4003_POS_RF4
SA003606LPDAY4005_POS_RF4
SA003607LPDAY4004_POS_RF4
SA003608LPDAY8001_POS_RF8
SA003609LPDAY8004_POS_RF8
SA003610LPDAY8005_POS_RF8
SA003611LPDAY8003_POS_RF8
SA003612LPDAY8002_POS_RF8
SA003613LI_422_POS_RFChow 4
SA003614LI_423_POS_RFChow 4
SA003615LI_421_POS_RFChow 4
SA003616LI_420_POS_RFChow 4
SA003617LI_419_POS_RFChow 4
SA003618LI_418_POS_RFChow 4
SA003619LI_8 39_POS_RFChow 8
SA003620LI_8 37_POS_RFChow 8
SA003621LI_8 40_POS_RFChow 8
SA003622LI_8 42_POS_RFChow 8
SA003623LI_8 36_POS_RFChow 8
SA003624LI_8 41_POS_RFChow 8
SA003625LI_8 38_POS_RFChow 8
SA003626LC_1_POS_RFChow uninfected
SA003627LC_4_POS_RFChow uninfected
SA003628LC_5_POS_RFChow uninfected
SA003629LC_6_POS_RFChow uninfected
SA003630LC_2_POS_RFChow uninfected
SA003631LC_3_POS_RFChow uninfected
SA003632LI_435_POS_RFHF 4
SA003633LI_430_POS_RFHF 4
SA003634LI_432_POS_RFHF 4
SA003635LI_431_POS_RFHF 4
SA003636LI_433_POS_RFHF 4
SA003637LI_434_POS_RFHF 4
SA003638LI_8 51_POS_RFHF 8
SA003639LI_8 53_POS_RFHF 8
SA003640LI_8 55_POS_RFHF 8
SA003641LI_8 50_POS_RFHF 8
SA003642LI_8 54_POS_RFHF 8
SA003643LI_8 52_POS_RFHF 8
SA003644LC_16_POS_RFHF uninfected
SA003645LC_15_POS_RFHF uninfected
SA003646LC_13_POS_RFHF uninfected
SA003647LC_12_POS_RFHF uninfected
SA003648LC_17_POS_RFHF uninfected
SA003649LC_14_POS_RFHF uninfected
SA003650LI_424_POS_RFLF 4
SA003651LI_428_POS_RFLF 4
SA003652LI_427_POS_RFLF 4
SA003653LI_426_POS_RFLF 4
SA003654LI_425_POS_RFLF 4
SA003655LI_429_POS_RFLF 4
SA003656LI_8 44_POS_RFLF 8
SA003657LI_8 47_POS_RFLF 8
SA003658LI_8 48_POS_RFLF 8
SA003659LI_8 45_POS_RFLF 8
SA003660LI_8 43_POS_RFLF 8
SA003661LI_8 46_POS_RFLF 8
SA003662LI_8 49_POS_RFLF 8
SA003663LC_8_POS_RFLF uninfected
SA003664LC_7_POS_RFLF uninfected
SA003665LC_10_POS_RFLF uninfected
SA003666LC_11_POS_RFLF uninfected
SA003667LC_9_POS_RFLF uninfected
SA003668LPCTRL001_POS_RFuninfected
SA003669LPCTRL002_POS_RFuninfected
SA003670LPCTRL003_POS_RFuninfected
SA003671LPCTRL004_POS_RFuninfected
SA003672LPCTRL005_POS_RFuninfected
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Collection:

Collection ID:CO000071
Collection Summary:-
Sample Type:Tissue
Storage Conditions:-80

Treatment:

Treatment ID:TR000089

Sample Preparation:

Sampleprep ID:SP000084
Sampleprep Summary:Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down and reconstituted in 100uL of 95:5 water:methanol.
Sampleprep Protocol Filename:RTI__BECK-LUNG_DIET_Metabolomics_Procedure.docx
Processing Method:Homogenization
Extraction Method:Acetonitrile
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5
Organ:Lung

Combined analysis:

Analysis ID AN000107 AN000108
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um) Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 Waters Synapt G2
Ion Mode POSITIVE NEGATIVE
Units Normalized abundance Normalized abundance

Chromatography:

Chromatography ID:CH000075
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Pressure:6000-10000
Column Temperature:50 C
Flow Gradient:Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6
Flow Rate:0.4 mL/min
Injection Temperature:8 C
Internal Standard:L-Tryptophan-d5
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Analytical Time:22 min
Weak Wash Solvent Name:5%MeOH
Weak Wash Volume:1000 uL
Strong Wash Solvent Name:80%MeOH
Strong Wash Volume:1000 uL
Target Sample Temperature:8 C
Sample Loop Size:10 uL
Sample Syringe Size:100 uL
Randomization Order:Yes
Chromatography Type:Reversed phase

MS:

MS ID:MS000083
Analysis ID:AN000107
Instrument Name:Waters Synapt G2
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
Capillary Temperature:110 C
Capillary Voltage:3.2 kV
Collision Energy:4
Helium Flow:180
Ionization:ES+
Mass Accuracy:10 ppm
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/s
Scanning Cycle:1 s
Tube Lens Voltage:75
  
MS ID:MS000084
Analysis ID:AN000108
Instrument Name:Waters Synapt G2
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Capillary Temperature:110 C
Capillary Voltage:2.8 kV
Collision Energy:4
Helium Flow:180
Ionization:ES-
Source Temperature:110 C
Dataformat:Continuum
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/z
Scanning Cycle:1 s
Tube Lens Voltage:74
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