Summary of Study ST000070
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000066. The data can be accessed directly via it's Project DOI: 10.21228/M8QG6H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000070 |
| Study Title | Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice (via tissue) |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-06-14 |
| Num Groups | 4 |
| Total Subjects | 22 |
| Raw Data Available | No |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-07-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000066 |
| Project DOI: | doi: 10.21228/M8QG6H |
| Project Title: | Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice |
| Project Type: | Effects and role of obesity on the severity of influenza |
| Project Summary: | During the 2009 H1N1 influenza pandemic outbreak, obese individuals were reported to be at greater risk for morbidity and mortality from pandemic infection. However, the mechanisms contributing to greater influenza severity in infected obese individuals remain unclear. Given that one in ten individuals is obese, and worldwide influenza outbreaks are a consistent public health burden, garnering a better understanding of the pathways and mechanisms contributing to greater influenza severity in the obese is essential for limiting influenza infection mortality in this at-risk population. Closely paralleling pH1N1 infection outcome in humans, obese mice exhibit increased morbidity and mortality following pH1N1 infection. In mice, obesity impairs the function of natural killer cells, dendritic cells, macrophage, B cells and memory T cells. Further, several analyses of lung antiviral responses revealed that obese mice have greater lung damage, lung immune cell infiltration and impaired lung healing after infection. Nevertheless, it remains unclear how altered immune cell function contributes to greater lung damage and increased infection severity in obese mice. Metabolomics will be used to dissect the metabolic consequences of obesity on the immune response to pH1N1 infection. We will compare metabolic profiles of lung-specific and peripheral samples from uninfected and infected lean and obese mice during early and late phases of influenza immunity. |
| Institute: | University of North Carolina at Chapel Hill |
| Department: | Department of Nutrition |
| Last Name: | Beck |
| First Name: | Melinda |
| Address: | 2303 MHRB, CB #7461, UNC, Chapel Hill NC 27599 |
| Email: | melinda_beck@unc.edu |
| Phone: | 919-966-6809 |
Subject:
| Subject ID: | SU000089 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | approx. 15-16 weeks |
| Gender: | male and female |
| Animal Animal Supplier: | The Jackson Laboratory |
| Animal Feed: | high fat, low fat or normal chow |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Phenotype | Infection Status | Sex |
|---|---|---|---|---|
| SA003673 | SD_02242014_RP_LPALL0002 | -- | -- | -- |
| SA003674 | SD_02242014_RP_LPALL0001 | -- | -- | -- |
| SA003675 | SD_02242014_RP_LPALL0003 | -- | -- | -- |
| SA003676 | SD_02242014_RP_LLI8_67 | lean | D8 infected | f |
| SA003677 | SD_02242014_RP_LLI8_92 | lean | D8 infected | f |
| SA003678 | SD_02242014_RP_LLI8_68 | lean | D8 infected | f |
| SA003679 | SD_02242014_RP_LLI8_65 | lean | D8 infected | m |
| SA003680 | SD_02242014_RP_LLI8_86 | lean | D8 infected | m |
| SA003681 | SD_02242014_RP_LLI8_94 | lean | D8 infected | m |
| SA003682 | SD_02242014_RP_LLPD8002 | lean | infected | -- |
| SA003683 | SD_02242014_RP_LLPD8001 | lean | infected | -- |
| SA003684 | SD_02242014_RP_LLPD8003 | lean | infected | -- |
| SA003685 | SD_02242014_RP_LLPCTRL002 | lean | uninfected | -- |
| SA003686 | SD_02242014_RP_LLPCTRL001 | lean | uninfected | -- |
| SA003687 | SD_02242014_RP_LLPCTRL003 | lean | uninfected | -- |
| SA003688 | SD_02242014_RP_LLC_56 | lean | uninfected | f |
| SA003689 | SD_02242014_RP_LLC_57 | lean | uninfected | f |
| SA003690 | SD_02242014_RP_LLC_40 | lean | uninfected | f |
| SA003691 | SD_02242014_RP_LLC_36 | lean | uninfected | m |
| SA003692 | SD_02242014_RP_LLC_47 | lean | uninfected | m |
| SA003693 | SD_02242014_RP_OLC_51 | lean | uninfected | m |
| SA003695 | SD_02242014_RP_OLI8_91 | obese | D8 infected | f |
| SA003696 | SD_02242014_RP_OLI8_74 | obese | D8 infected | f |
| SA003697 | SD_02242014_RP_OLI8_75 | obese | D8 infected | f |
| SA003698 | SD_02242014_RP_OLI8_88 | obese | D8 infected | m |
| SA003699 | SD_02242014_RP_OLI8_109 | obese | D8 infected | m |
| SA003700 | SD_02242014_RP_OLPD8003 | obese | infected | -- |
| SA003701 | SD_02242014_RP_OLPD8001 | obese | infected | -- |
| SA003702 | SD_02242014_RP_OLPD8002 | obese | infected | -- |
| SA003703 | SD_02242014_RP_OLPCTRL002 | obese | uninfected | -- |
| SA003704 | SD_02242014_RP_OLPCTRL001 | obese | uninfected | -- |
| SA003705 | SD_02242014_RP_OLPCTRL003 | obese | uninfected | -- |
| SA003706 | SD_02242014_RP_OLC_41 | obese | uninfected | f |
| SA003707 | SD_02242014_RP_OLC_44 | obese | uninfected | f |
| SA003708 | SD_02242014_RP_OLC_63 | obese | uninfected | f |
| SA003694 | SD_02242014_RP_OLC_53 | obese | uninfected | m |
| SA003709 | SD_02242014_RP_OLC_35 | obese | uninfected | m |
| Showing results 1 to 37 of 37 |
Collection:
| Collection ID: | CO000072 |
| Collection Summary: | - |
| Sample Type: | Tissue |
| Storage Conditions: | -80 |
Treatment:
| Treatment ID: | TR000090 |
Sample Preparation:
| Sampleprep ID: | SP000085 |
| Sampleprep Summary: | Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down and reconstituted in 100uL of 95:5 water:methanol. |
| Sampleprep Protocol Filename: | RTI_BECK_Lung-Genetic_RP_Metabolomics_Procedure.docx |
| Processing Method: | Homogenization |
| Extraction Method: | Acetonitrile |
| Extract Storage: | -80 C |
| Sample Resuspension: | 95:5 Water:Methanol |
| Sample Spiking: | L-Tryptophan-d5 |
| Organ: | Lung |
Combined analysis:
| Analysis ID | AN000109 | AN000110 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 | Waters Synapt G2 |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized abundance | Normalized abundance |
Chromatography:
| Chromatography ID: | CH000076 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Pressure: | 6000-10000 |
| Column Temperature: | 50 C |
| Flow Gradient: | Time(min) Flow Rate %A %B Curve ; 1. Initial 0.400 100.0 0.0 ; 2. 1.00 0.400 100.0 0.0 6 ; 3. 16.00 0.400 0.0 100.0 6 ; 4. 20.00 0.400 0.0 100.0 6 ; 5. 22.00 0.400 100.0 0.0 6 |
| Flow Rate: | 0.4 mL/min |
| Injection Temperature: | 8 C |
| Internal Standard: | L-Tryptophan-d5 |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Analytical Time: | 22 min |
| Weak Wash Solvent Name: | 5%MeOH |
| Weak Wash Volume: | 1000 uL |
| Strong Wash Solvent Name: | 80%MeOH |
| Strong Wash Volume: | 1000 uL |
| Target Sample Temperature: | 8 C |
| Sample Loop Size: | 10 uL |
| Sample Syringe Size: | 100 uL |
| Randomization Order: | Yes |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000085 |
| Analysis ID: | AN000109 |
| Instrument Name: | Waters Synapt G2 |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 110 C |
| Capillary Voltage: | 3.2 kV |
| Collision Energy: | 4 |
| Fragmentation Method: | CID |
| Helium Flow: | 180 |
| Ionization: | ES+ |
| Mass Accuracy: | 10 ppm |
| Source Temperature: | 110 C |
| Dataformat: | Continuum |
| Desolvation Gas Flow: | 400 L/Hr |
| Desolvation Temperature: | 400 C |
| Resolution Setting: | 18000 |
| Scan Range Moverz: | 50-1000 m/s |
| Scanning Cycle: | 1 s |
| Tube Lens Voltage: | 75 |
| MS ID: | MS000086 |
| Analysis ID: | AN000110 |
| Instrument Name: | Waters Synapt G2 |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 110 C |
| Capillary Voltage: | 2.8 kV |
| Collision Energy: | 4 |
| Fragmentation Method: | CID |
| Helium Flow: | 180 |
| Ionization: | ES- |
| Source Temperature: | 110 C |
| Dataformat: | Continuum |
| Desolvation Gas Flow: | 400 L/Hr |
| Desolvation Temperature: | 400 C |
| Resolution Setting: | 18000 |
| Scan Range Moverz: | 50-1000 m/z |
| Scanning Cycle: | 1 s |
| Tube Lens Voltage: | 74 |
