Summary of Study ST000071

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000066. The data can be accessed directly via it's Project DOI: 10.21228/M8QG6H This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000071
Study TitleMetabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice (via Urine)
University of North Carolina
DepartmentSystems and Translational Sciences
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Submit Date2014-06-14
Num Groups4
Total Subjects22
Raw Data AvailableNo
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2015-07-31
Release Version1
Susan Sumner Susan Sumner application/zip

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Project ID:PR000066
Project DOI:doi: 10.21228/M8QG6H
Project Title:Metabolomic profiling of influenza: a 2009 pandemic H1N1 influenza in lean and obese mice
Project Type:Effects and role of obesity on the severity of influenza
Project Summary:During the 2009 H1N1 influenza pandemic outbreak, obese individuals were reported to be at greater risk for morbidity and mortality from pandemic infection. However, the mechanisms contributing to greater influenza severity in infected obese individuals remain unclear. Given that one in ten individuals is obese, and worldwide influenza outbreaks are a consistent public health burden, garnering a better understanding of the pathways and mechanisms contributing to greater influenza severity in the obese is essential for limiting influenza infection mortality in this at-risk population. Closely paralleling pH1N1 infection outcome in humans, obese mice exhibit increased morbidity and mortality following pH1N1 infection. In mice, obesity impairs the function of natural killer cells, dendritic cells, macrophage, B cells and memory T cells. Further, several analyses of lung antiviral responses revealed that obese mice have greater lung damage, lung immune cell infiltration and impaired lung healing after infection. Nevertheless, it remains unclear how altered immune cell function contributes to greater lung damage and increased infection severity in obese mice. Metabolomics will be used to dissect the metabolic consequences of obesity on the immune response to pH1N1 infection. We will compare metabolic profiles of lung-specific and peripheral samples from uninfected and infected lean and obese mice during early and late phases of influenza immunity.
Institute:University of North Carolina at Chapel Hill
Department:Department of Nutrition
Last Name:Beck
First Name:Melinda
Address:2303 MHRB, CB #7461, UNC, Chapel Hill NC 27599


Subject ID:SU000090
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:approx. 15-16 weeks
Gender:male and female
Animal Animal Supplier:The Jackson Laboratory
Animal Feed:high fat, low fat or normal chow
Species Group:Mammal


Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Phenotype Infection Status Sex
SA003710UPALL0003-- -- --
SA003711UPALL0002-- -- --
SA003712UPALL0001-- -- --
SA003713LUI2_68 lean D2 Infected f
SA003714LUI2_67 lean D2 Infected f
SA003715LUI2_92 lean D2 Infected f
SA00371994lean D8 infected m
SA00372086lean D8 infected m
SA00372165lean D8 infected m
SA003716LUC_67 lean uninfected f
SA003717LUC_68 lean uninfected f
SA003718LUC_92 lean uninfected f
SA00372236lean uninfected m
SA00372351lean uninfected m
SA00372447lean uninfected m
SA003725OUI2_74 obese D2 Infected f
SA003726OUI2_75 obese D2 Infected f
SA003727OUI2_91 obese D2 Infected f
SA003732109obese D8 infected m
SA00373388obese D8 infected m
SA003728OUC_74 obese uninfected f
SA003729OUC_91 obese uninfected f
SA003730OUC_75 obese uninfected f
SA00373435obese uninfected m
SA00373153obese uninfected m
Showing results 1 to 25 of 25


Collection ID:CO000073
Collection Summary:-
Sample Type:Tissue
Storage Conditions:-80


Treatment ID:TR000091

Sample Preparation:

Sampleprep ID:SP000086
Sampleprep Summary:Samples were homogenized on a baed beater at1750rpm in 10uL of 50:50 acetonitrile:water per 1 mg of tissue using washed ceramic beads. Extraction was performed using acetonitrile. Samples were dried down and reconstituted in 100uL of 95:5 water:methanol.
Sampleprep Protocol Filename:RTI_BECK_Urine-Genetic_HILIC_Metabolomics_Procedure.docx
Processing Method:Homogenization
Extraction Method:Acetonitrile
Extract Storage:-80 C
Sample Resuspension:95:5 Water:Methanol
Sample Spiking:L-Tryptophan-d5

Combined analysis:

Analysis ID AN000111 AN000112
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Waters Acquity Waters Acquity
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS instrument type QTOF QTOF
MS instrument name Waters Synapt G2 QTOF Waters Synapt G2 QTOF
Units Normalized abundance Normalized abundance


Chromatography ID:CH000077
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Pressure:6000-10000
Column Temperature:40 C
Flow Gradient:Time(min) Flow Rate %A %B: 1. Initial 0.400 99.0 1.0; 2. 1.00 0.400 99.0 1.0; 3. 12.00 0.400 1.0 99.0; 4. 14.00 0.400 1.0 99.0; 5. 15.00 0.400 99.0 1.0; 6. 19.00 0.400 99.0 1.0
Flow Rate:0.4 mL/min
Injection Temperature:8 C
Internal Standard:L-Tryptophan-d5
Solvent A:50% acetonitrile/50% water; 0.1% formic acid; 10 mM ammonium acetate
Solvent B:50% acetonitrile/50% water; 0.1% formic acid in 10 mM ammonium acetate
Analytical Time:19 min
Weak Wash Solvent Name:5%MeOH
Weak Wash Volume:1000 uL
Strong Wash Solvent Name:80%MeOH
Strong Wash Volume:1000 uL
Target Sample Temperature:8 C
Sample Loop Size:10 uL
Sample Syringe Size:100 uL
Randomization Order:Yes
Chromatography Type:HILIC


MS ID:MS000087
Analysis ID:AN000111
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
Capillary Temperature:110 C
Capillary Voltage:3.2 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Mass Accuracy:10 ppm
Source Temperature:110 C
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/s
Scanning Cycle:1 s
Tube Lens Voltage:75
MS ID:MS000088
Analysis ID:AN000112
Instrument Name:Waters Synapt G2 QTOF
Instrument Type:QTOF
Capillary Temperature:110 C
Capillary Voltage:2.8 kV
Collision Energy:4
Fragmentation Method:CID
Helium Flow:180
Source Temperature:110 C
Desolvation Gas Flow:400 L/Hr
Desolvation Temperature:400 C
Resolution Setting:18000
Scan Range Moverz:50-1000 m/z
Scanning Cycle:1 s
Tube Lens Voltage:74