Summary of Study ST000095
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000087. The data can be accessed directly via it's Project DOI: 10.21228/M8F30Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000095 |
Study Title | Dysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome |
Study Type | Chemical dosage and feeding study |
Study Summary | Targeted metabolomic analysis of bile acids was performed on 15 mouse liver samples collected from mice euthanized at 9 months following consumption of a high fat diet w/o perinatal DDT exposure. Funded by the National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization. |
Institute | University of California, Davis |
Department | U.S.D.A. Western Human Nutrition Research Center |
Laboratory | Newman |
Last Name | Newman |
First Name | John |
Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
john.newman@ars.usda.gov | |
Phone | +1-530-752-1009 |
Submit Date | 2014-07-10 |
Num Groups | 2 |
Total Subjects | 15 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Uploaded File Size | 23 M |
Analysis Type Detail | LC-MS |
Release Date | 2015-02-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000087 |
Project DOI: | doi: 10.21228/M8F30Z |
Project Title: | Dysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome |
Project Type: | Animal chemical exposure and feeding study |
Project Summary: | This study evaluated the effect of perinatal DDT exposure on metabolic syndrome in mice exposed to doses that mimicked human exposure. The samples tested were from 2 groups: 1) a group with perinatal exposure to DDT consuming a high fat diet (DDHF), 2) a control group consuming a high fat diet (CHF). Results indicated the DDHF group had comparatively elevated plasma concentrations of glucose, insulin, cholesterol, and triglycerides, CYP7A1 gene expression, homeostasis model assessment- insulin resistance (HOMA-IR), and fat mass (%), as well as reduced thermogenesis. These data suggest perinatal DDT exposure may cause the co-occurrence of conditions associated with metabolic syndrome, including glucose intolerance, hyperinsulinemia, and dyslipidemia, impaired thermogenesis, and obesity. |
Institute: | University of California, Davis |
Department: | Environmental Toxicology |
Laboratory: | La Merrill |
Last Name: | La Merrill |
First Name: | Michele |
Address: | 1 Shields Ave, Davis CA |
Email: | mlamerrill@ucdavis.edu |
Phone: | +1-530-754-7254 |
Funding Source: | National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D |
Subject:
Subject ID: | SU000114 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 9 months |
Gender: | Female |
Animal Housing: | Singly housed |
Animal Feed: | High fat diet (HFD, 4.73 kcal/g, 20% protein, 35% carbohydrate, and 45% fat per kcal; D12451, Research Diets, New Brunswick, NJ) |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment |
---|---|---|
SA005396 | T43-1 | DDT |
SA005397 | T26-6 | DDT |
SA005398 | T31-6 | DDT |
SA005399 | T52-6 | DDT |
SA005400 | T36-4 | DDT |
SA005401 | T12-4 | DDT |
SA005402 | T30-2 | DDT |
SA005403 | T18-3 | DDT |
SA005404 | V78-6 | OIL |
SA005405 | V38-9 | OIL |
SA005406 | V47-6 | OIL |
SA005407 | V77-5 | OIL |
SA005408 | V44-2 | OIL |
SA005409 | V69-3 | OIL |
SA005410 | V32-6 | OIL |
Showing results 1 to 15 of 15 |
Collection:
Collection ID: | CO000097 |
Collection Summary: | - |
Collection Protocol Filename: | Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf |
Sample Type: | Liver tissue |
Collection Method: | Dissection |
Storage Conditions: | - 80°C |
Storage Vials: | Eppendorf tubes |
Treatment:
Treatment ID: | TR000115 |
Treatment Summary: | 1.7 mg DDT/kg body weight was administered to dams daily on gestational day 11 through postnatal day 5 and litters were culled to 6 mice at postnatal day 6. Mice were metabolically screened from 2-6 months when they consumed a high fat diet. Mice were sacrificed at 9 months of age. |
Treatment Protocol Filename: | Newman_Lab_Bile_Acid_Protocols_La_Merrill_WCMC_P&F.pdf |
Treatment: | Intervention |
Treatment Compound: | p,p`-DDT, o,p`-DDT |
Treatment Route: | Oral |
Treatment Dose: | 77.2% p,p`-DDT (98.5% purity neat, AccuStandard, New Haven, CT) and 22.8% o,p`-DDT (100% purity neat, AccuStandard) were dissolved at a final concentration of 0.17 g DDT mixture/L organic olive oil |
Treatment Doseduration: | From 11.5 days post coitus to postnatal day 5 |
Treatment Vehicle: | Olive oil |
Animal Endp Euthanasia: | 9 months |
Animal Endp Tissue Coll List: | Liver |
Animal Endp Tissue Proc Method: | Dissected, placed on dry ice |
Sample Preparation:
Sampleprep ID: | SP000110 |
Sampleprep Summary: | A 15 mg liver sample (n=1 female/litter in 7 VEH and 8 DDT litters) was pulverized on dry ice, enriched with deuterated bile acid surrogates, butylated hydroxytoluene and ethylinediaminetetraacetic acid, and extracted twice with 500 uL methanol. The combined extract was dried, reconstituted in 100uL 50:50 methanol:acetonitrile with internal standards 1-phenyl-3-hexanoic acid urea and 1-cyclohexyl-3-dodecanoic acid urea (Sigma-Aldrich, St. Louis, MO) and filtered at 0.1 µm. |
Sampleprep Protocol Filename: | Newman_Lab_Bile_Acid_Protocols_La_Merrill_WCMC_P&F.pdf |
Processing Method: | Pulverization on dry ice |
Extraction Method: | Methanol |
Extract Concentration Dilution: | Dried with Rotoevaporator |
Extract Storage: | Placed in 10 °C autosampler and immediately analyzed |
Sample Spiking: | Spiked with deuterated bile acid surrogates. |
Organ: | Liver |
Combined analysis:
Analysis ID | AN000151 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity UPLC |
Column | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex API 6500 QTrap |
Ion Mode | NEGATIVE |
Units | % abundance |
Chromatography:
Chromatography ID: | CH000107 |
Chromatography Summary: | Targeted UPLC-MS/MS method |
Methods Filename: | Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf |
Instrument Name: | Waters Acquity UPLC |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 60 C |
Flow Gradient: | Yes |
Flow Rate: | 0.4mL/min |
Internal Standard: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 16 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000127 |
Analysis ID: | AN000151 |
Instrument Name: | ABI Sciex API 6500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Ion Source Temperature: | See protocol/methods file |
Ion Spray Voltage: | See protocol/methods file |