Summary of Study ST000107
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000046. The data can be accessed directly via it's Project DOI: 10.21228/M84S36 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST000107 |
Study Title | Metabolomic and lipidomic profiles in response to exogenous insulin and GLP-1 infusions during prolonged fasting |
Study Type | Timecourse |
Study Summary | Seventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes. |
Institute | University of California, Davis |
Department | Nutrition |
Laboratory | Newman |
Last Name | Newman |
First Name | John |
Address | 430 W. Health Sciences Dr., Davis, CA 95616 |
john.newman@ars.usda.gov | |
Phone | +1-530-752-1009 |
Submit Date | 2014-07-10 |
Num Groups | 4 |
Total Subjects | 5 |
Raw Data Available | Yes |
Raw Data File Type(s) | wiff |
Uploaded File Size | 751 M |
Analysis Type Detail | GC-MS/LC-MS |
Release Date | 2015-02-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR000046 |
Project DOI: | doi: 10.21228/M84S36 |
Project Title: | Metabolomic & lipidomic profiles in response to exogenous insulin & GLP-1 infusions during prolonged fasting |
Project Type: | Timecourse |
Project Summary: | This application requests funding to access state-of-the-art metabolomics and lipidomic platforms at the NIH West Coast Metabolomics Center to analyze plasma samples from recent insulin and glucagon-like peptide-1 (GLP-1) infusion experiments performed in prolong-fasted elephant seals. This suite of studies was designed to better assess the mechanisms contributing to the onset of an insulin resistantlike condition induced by prolonged food deprivation/starvation in mammals. Because elephant seals have evolved robust physiological mechanisms that have allowed them to naturally tolerate such protracted bouts of fasting, they provide an ideal model to address our central hypothesis that increased lipid utilization late in the fast contributes to insulin resistance in elephant seals. Insulin resistance is a common consequence of fasting in mammals and, while the mechanisms by which it manifests are still unclear, a metabolic shift favoring increased mobilization and utilization of lipids during prolonged food deprivation may be a principal causative factor. Insulin resistance has a negative connotation due to its association with obesity and diabetes among humans, but it has been suggested to be an adaptive response to food deprivation. |
Institute: | University of California, Merced |
Department: | School of Natural Sciences |
Laboratory: | Molecular and Cellular Biology, Natural Sciences |
Last Name: | Ortiz |
First Name: | Rudy |
Address: | 5200 N. Lake Rd.; Merced, CA 95343 |
Email: | rortiz@ucmerced.edu |
Phone: | 209.228.2964 |
Subject:
Subject ID: | SU000126 |
Subject Type: | Animal |
Subject Species: | Mirounga angustirostris |
Taxonomy ID: | 9716 |
Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mirounga angustirostris (Factor headings shown in green)
mb_sample_id | local_sample_id | Treatment | Timepoint |
---|---|---|---|
SA005921 | IE05-0 | Early ITT Infusion Study | 0 mins |
SA005922 | IE04-0 | Early ITT Infusion Study | 0 mins |
SA005923 | IE03-0 | Early ITT Infusion Study | 0 mins |
SA005924 | IE02-0 | Early ITT Infusion Study | 0 mins |
SA005925 | IE01-0 | Early ITT Infusion Study | 0 mins |
SA005926 | IE05-10 | Early ITT Infusion Study | 10 mins |
SA005927 | IE04-10 | Early ITT Infusion Study | 10 mins |
SA005928 | IE01-10 | Early ITT Infusion Study | 10 mins |
SA005929 | IE03-10 | Early ITT Infusion Study | 10 mins |
SA005930 | IE02-10 | Early ITT Infusion Study | 10 mins |
SA005931 | IE03-120 | Early ITT Infusion Study | 120 mins |
SA005932 | IE02-120 | Early ITT Infusion Study | 120 mins |
SA005933 | IE04-120 | Early ITT Infusion Study | 120 mins |
SA005934 | IE05-120 | Early ITT Infusion Study | 120 mins |
SA005935 | IE01-120 | Early ITT Infusion Study | 120 mins |
SA005936 | IE01-30 | Early ITT Infusion Study | 30 mins |
SA005937 | IE02-30 | Early ITT Infusion Study | 30 mins |
SA005938 | IE03-30 | Early ITT Infusion Study | 30 mins |
SA005939 | IE04-30 | Early ITT Infusion Study | 30 mins |
SA005940 | IE05-30 | Early ITT Infusion Study | 30 mins |
SA005941 | IE04-60 | Early ITT Infusion Study | 60 mins |
SA005942 | IE01-60 | Early ITT Infusion Study | 60 mins |
SA005943 | IE03-60 | Early ITT Infusion Study | 60 mins |
SA005944 | IE02-60 | Early ITT Infusion Study | 60 mins |
SA005948 | C01-0 | GLP1/Late GTT | 0 mins |
SA005949 | C02-0 | GLP1/Late GTT | 0 mins |
SA005950 | A01-0 | GLP1/Late GTT | 0 mins |
SA005951 | B03-0 | GLP1/Late GTT | 0 mins |
SA005952 | C03-0 | GLP1/Late GTT | 0 mins |
SA005953 | C04-0 | GLP1/Late GTT | 0 mins |
SA005954 | B01-0 | GLP1/Late GTT | 0 mins |
SA005955 | B02-0 | GLP1/Late GTT | 0 mins |
SA005956 | A04-0 | GLP1/Late GTT | 0 mins |
SA005957 | A02-0 | GLP1/Late GTT | 0 mins |
SA005958 | A03-0 | GLP1/Late GTT | 0 mins |
SA005959 | A05-0 | GLP1/Late GTT | 0 mins |
SA005960 | A01-10 | GLP1/Late GTT | 10 mins |
SA005961 | B01-10 | GLP1/Late GTT | 10 mins |
SA005962 | A03-10 | GLP1/Late GTT | 10 mins |
SA005963 | C03-10 | GLP1/Late GTT | 10 mins |
SA005964 | A02-10 | GLP1/Late GTT | 10 mins |
SA005965 | A04-10 | GLP1/Late GTT | 10 mins |
SA005966 | A05-10 | GLP1/Late GTT | 10 mins |
SA005967 | C02-10 | GLP1/Late GTT | 10 mins |
SA005968 | B02-10 | GLP1/Late GTT | 10 mins |
SA005969 | C04-10 | GLP1/Late GTT | 10 mins |
SA005970 | C01-10 | GLP1/Late GTT | 10 mins |
SA005971 | B03-10 | GLP1/Late GTT | 10 mins |
SA005972 | A02-120 | GLP1/Late GTT | 120 mins |
SA005973 | A03-120 | GLP1/Late GTT | 120 mins |
SA005974 | C04-120 | GLP1/Late GTT | 120 mins |
SA005975 | A01-120 | GLP1/Late GTT | 120 mins |
SA005976 | A04-120 | GLP1/Late GTT | 120 mins |
SA005977 | B01-120 | GLP1/Late GTT | 120 mins |
SA005978 | C03-120 | GLP1/Late GTT | 120 mins |
SA005979 | C01-120 | GLP1/Late GTT | 120 mins |
SA005980 | C02-120 | GLP1/Late GTT | 120 mins |
SA005981 | B03-120 | GLP1/Late GTT | 120 mins |
SA005982 | A05-120 | GLP1/Late GTT | 120 mins |
SA005983 | B02-120 | GLP1/Late GTT | 120 mins |
SA005984 | A05-30 | GLP1/Late GTT | 30 mins |
SA005985 | B01-30 | GLP1/Late GTT | 30 mins |
SA005986 | A03-30 | GLP1/Late GTT | 30 mins |
SA005987 | A04-30 | GLP1/Late GTT | 30 mins |
SA005988 | A02-30 | GLP1/Late GTT | 30 mins |
SA005989 | A01-30 | GLP1/Late GTT | 30 mins |
SA005990 | C04-30 | GLP1/Late GTT | 30 mins |
SA005991 | C01-30 | GLP1/Late GTT | 30 mins |
SA005992 | C02-30 | GLP1/Late GTT | 30 mins |
SA005993 | B03-30 | GLP1/Late GTT | 30 mins |
SA005994 | C03-30 | GLP1/Late GTT | 30 mins |
SA005995 | B02-30 | GLP1/Late GTT | 30 mins |
SA005996 | C03-60 | GLP1/Late GTT | 60 mins |
SA005997 | C02-60 | GLP1/Late GTT | 60 mins |
SA005998 | C01-60 | GLP1/Late GTT | 60 mins |
SA005999 | A02-60 | GLP1/Late GTT | 60 mins |
SA006000 | A04-60 | GLP1/Late GTT | 60 mins |
SA006001 | A03-60 | GLP1/Late GTT | 60 mins |
SA006002 | A01-60 | GLP1/Late GTT | 60 mins |
SA006003 | B02-60 | GLP1/Late GTT | 60 mins |
SA006004 | B03-60 | GLP1/Late GTT | 60 mins |
SA006005 | A05-60 | GLP1/Late GTT | 60 mins |
SA006006 | C04-60 | GLP1/Late GTT | 60 mins |
SA006007 | B01-60 | GLP1/Late GTT | 60 mins |
SA006008 | IL05-0 | Late ITT Infusion Study | 0 mins |
SA006009 | IL03-0 | Late ITT Infusion Study | 0 mins |
SA006010 | IL02-0 | Late ITT Infusion Study | 0 mins |
SA006011 | IL01-0 | Late ITT Infusion Study | 0 mins |
SA006012 | IL04-0 | Late ITT Infusion Study | 0 mins |
SA006013 | IL03-10 | Late ITT Infusion Study | 10 mins |
SA006014 | IL04-10 | Late ITT Infusion Study | 10 mins |
SA006015 | IL01-10 | Late ITT Infusion Study | 10 mins |
SA006016 | IL02-10 | Late ITT Infusion Study | 10 mins |
SA006017 | IL05-10 | Late ITT Infusion Study | 10 mins |
SA006018 | IL01-120 | Late ITT Infusion Study | 120 mins |
SA006019 | IL02-120 | Late ITT Infusion Study | 120 mins |
SA006020 | IL05-120 | Late ITT Infusion Study | 120 mins |
SA006021 | IL03-120 | Late ITT Infusion Study | 120 mins |
SA006022 | IL04-120 | Late ITT Infusion Study | 120 mins |
SA006023 | IL05-30 | Late ITT Infusion Study | 30 mins |
Collection:
Collection ID: | CO000110 |
Collection Summary: | Following the GLP-1 or insulin infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. |
Collection Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Sample Type: | Blood |
Collection Time: | 10, 30, 60, 90, and 120 minutes postprandial |
Storage Conditions: | -80 C |
Blood Serum Or Plasma: | Plasma |
Treatment:
Treatment ID: | TR000128 |
Treatment Summary: | Seventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes. |
Treatment Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Treatment Compound: | GLP-1 or Insulin |
Treatment Route: | Infusion |
Treatment Dose: | low (10 pmol/kg) or high (100 pmol/kg) dose of GLP-1, insulin (0.065 U/kg) |
Animal Fasting: | Yes, for insulin study |
Sample Preparation:
Sampleprep ID: | SP000123 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Processing Storage Conditions: | - 20 °C |
Extraction Method: | SPE |
Extract Concentration Dilution: | 250 µL |
Extract Cleanup: | SPE |
Extract Storage: | - 20 °C |
Sample Resuspension: | 100 µL |
Sample Spiking: | See sample prep protocol file |
Combined analysis:
Analysis ID | AN000177 | AN000178 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | GC | GC |
Chromatography system | ||
Column | ||
MS Type | ESI | EI |
MS instrument type | Triple quadrupole | Single quadrupole |
MS instrument name | ABI Sciex API 4000 QTrap | Agilent 5973N |
Ion Mode | POSITIVE | POSITIVE |
Units | nM | nM |
Chromatography:
Chromatography ID: | CH000121 |
Methods Filename: | Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf |
Column Temperature: | 60 C |
Flow Gradient: | See protocol/methods file |
Flow Rate: | 0.25 |
Internal Standard: | See protocol/methods file |
Retention Time: | See protocol/methods file |
Sample Injection: | 5 L |
Solvent A: | 100% water; 0.1% acetic acid |
Solvent B: | 90% acetonitrile/ 10% isopropanol |
Analytical Time: | 11 min |
Weak Wash Solvent Name: | 20% methanol, 10% isopropanol |
Weak Wash Volume: | 600 L |
Strong Wash Solvent Name: | 50:50 Acetonitrile:Methanol |
Strong Wash Volume: | 600 L |
Sample Loop Size: | 17 L |
Chromatography Type: | GC |
MS:
MS ID: | MS000140 |
Analysis ID: | AN000177 |
Instrument Name: | ABI Sciex API 4000 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | LC/MS |
Ion Mode: | POSITIVE |
Ion Source Temperature: | See protocol/methods file |
Ion Spray Voltage: | See protocol/methods file |
MS ID: | MS000141 |
Analysis ID: | AN000178 |
Instrument Name: | Agilent 5973N |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | LC/MS |
Ion Mode: | POSITIVE |
Ion Source Temperature: | See protocol/methods file |
Ion Spray Voltage: | See protocol/methods file |