Summary of study ST000246

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000246
Study TitleLipid Extraction Efficiency Comparison
Study TypeLipid Extraction Efficiency Comparison
Study SummaryAliquots of Jurkat T-lymphocyte cells were extracted using the Folch or Matyash method at different total volumes and run in triplicate to calculate the extraction efficiency for each method.
Institute
University of Florida
DepartmentDept. of Chemistry/SECIM
LaboratoryBiomedical Mass Spectrometry Lab
Last NameUlmer
First NameCandice
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailczulmer@chem.ufl.edu
Phone(352) 392-0515
Submit Date2015-09-15
Num Groups4
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s).raw
Uploaded File Size2.2 G
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Candice Ulmer Candice Ulmer
https://dx.doi.org/10.21228/M8DW2B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000125
Project DOI:doi: 10.21228/M8DW2B
Project Title:Mammalian Suspension-Cultured Cellular Metabolomics Workflow
Project Type:Metabolomics, Lipidomics, Untargeted
Project Summary:A workflow was optimized for the sample preparation of a single suspension-cultured cell pellet for both metabolomics and lipidomics analysis. Jurkat T-lymphocyte cells were washed with various rinsing solutions and the lipids extracted using different lipid extraction protocols to allow for the most reproducible and quantitative method.
Institute:University of Florida
Department:Dept. of Chemistry/SECIM
Laboratory:Biomedical Mass Spectrometry Lab
Last Name:Yost
First Name:Richard
Address:P.O. Box 117200, Gainesville, FL 32611
Email:ryost@aa.ufl.edu
Phone:(352) 392-0515
Funding Source:JDRF Research Grant
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